Department of Breast Oncology, Linyi Cancer Hospital , Linyi , Shandong , China.
Department of Breast Surgery, the affiliated hospital of Qingdao University , Qingdao , China.
Bioengineered. 2019 Dec;10(1):374-382. doi: 10.1080/21655979.2019.1657327.
The aim of the present study was to investigate the anticancer effects and potential mechanisms of polyphenol epigallocatechin-3-gallate (EGCG) on breast cancer MCF-7 cells and . Our results showed that EGCG significantly inhibited MCF-7 cell viability in a time- and dose-dependent manner. Flow cytometry analysis indicated that EGCG induced apoptosis and disrupted cell cycle progression at G2/M phase. Moreover, EGCG inhibited miR-25 expression and increased PARP, pro-caspase-3 and pro-caspase-9 at protein levels. Restoration of miR-25 inhibited EGCG-induced cell apoptosis. Furthermore, EGCG suppressed tumor growth by downregulating the expression of miR-25 and proteins associated with apoptosis, which was further confirmed by a reduction of Ki-67 and increase of pro-apoptotic PARP expression as determined by immunohistochemistry staining. These findings indicate that EGCG possesses chemopreventive potential in breast cancer which may serve as a promising anticancer agent for clinical applications.
本研究旨在探讨多酚表没食子儿茶素没食子酸酯（EGCG）对乳腺癌 MCF-7 细胞的抗癌作用及潜在机制。结果表明，EGCG 呈时间和剂量依赖性显著抑制 MCF-7 细胞活力。流式细胞术分析表明，EGCG 诱导细胞凋亡，并在 G2/M 期破坏细胞周期进程。此外，EGCG 抑制 miR-25 的表达，并增加 PARP、pro-caspase-3 和 pro-caspase-9 的蛋白水平。miR-25 的恢复抑制了 EGCG 诱导的细胞凋亡。此外，EGCG 通过下调 miR-25 的表达及其与凋亡相关的蛋白表达来抑制肿瘤生长，免疫组化染色进一步证实了这一点，Ki-67 的减少和促凋亡 PARP 表达的增加表明了这一点。这些发现表明，EGCG 具有预防乳腺癌的化学作用，可能成为临床应用有前途的抗癌药物。
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