Toledo Andrea Vanesa, de Remes Lenicov Ana M Marino, López Lastra Claudia C
Centro de Estudios Parasitológicos y de Vectores, UNLP-CONICET, Calle 2 Nro. 584, 1900 La Plata, Buenos Aires, Argentina.
Mycopathologia. 2007 Apr;163(4):225-32. doi: 10.1007/s11046-007-9006-y. Epub 2007 Apr 4.
Preliminary screening assays were carried out on 17 isolates from five fungal species Beauveria bassiana, Lecanicillium muscarium, Metarhizium anisopliae, Isaria farinosa, and I. fumosorosea. The three most effective isolates against Peregrinus maidis (Hemiptera: Delphacidae) were B. bassiana CEP 147, CEP 150, and CEP 189. There were no consistent differences found in males and females regarding fungal susceptibility. However, more females than males were proportionally infected. There was not a correlation between the percentage of conidial germination and the percentage of mortality caused by fungal infection in any of the treatments. Only B. bassiana CEP 147, which caused a cumulative mortality of 69.8 +/- 6.4% after 7 days post-inoculation, was selected to be assayed against adults of P. maidis, Delphacodes kuscheli (Hemiptera: Delphacidae), and Dalbulus maidis (Hemiptera: Cicadellidae). In pathogenicity tests significant differences were observed among treatments. After 2 weeks post-inoculation, both D. kuscheli (cumulative mortality of 73.3 +/- 9.0%) and P. maidis (cumulative mortality of 68.6 +/- 6.7%) were significantly more susceptible than D. maidis (cumulative mortality of 49.9 +/- 9.7%) to the selected isolate.
对来自球孢白僵菌、蜡蚧轮枝菌、绿僵菌、粉棒束孢和玫烟色棒束孢这五种真菌的17个分离株进行了初步筛选试验。对玉米蜡蝉（半翅目：飞虱科）最有效的三个分离株是球孢白僵菌CEP 147、CEP 150和CEP 189。在真菌易感性方面，未发现雄性和雌性之间存在一致差异。然而，按比例感染的雌性比雄性更多。在任何处理中，分生孢子萌发率与真菌感染引起的死亡率之间均无相关性。仅选择接种后7天累计死亡率为69.8±6.4%的球孢白僵菌CEP 147，对玉米蜡蝉成虫、库氏飞虱（半翅目：飞虱科）和玉米叶蝉（半翅目：叶蝉科）进行测定。在致病性试验中，各处理间观察到显著差异。接种后2周，库氏飞虱（累计死亡率为73.3±9.0%）和玉米蜡蝉（累计死亡率为68.6±6.7%）对所选分离株的易感性均显著高于玉米叶蝉（累计死亡率为49.9±9.7%）。
