选择性杀死转化的幼仓鼠肾（BHK）细胞。
Selective killing of transformed baby hamster kidney (BHK) cells.
作者信息
Pardee A B, James L J
出版信息
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4994-8. doi: 10.1073/pnas.72.12.4994.
Abstract
We report here that certain drugs can protect Syrian baby hamster kidney cells (BHK) in culture against the lethal agents cytosine arabinonucleoside, hydroxyurea, and colcemid. Polyoma virus-transformed BHK cells (PyBHK) are killed under the same conditions. The protective drugs include caffeine and streptovitacin A. Kinetic studies show that these drugs act specifically in G1, and that they shift BHK cells from G1 into the G0 state at the restriction point, similar to the effects of high cell density or serum deprivation. These drugs do not block the growth of PyBHK cells nearly as effectively, consistent with a reduced effectiveness of restriction point control in virus-transformed cells. Consequently, the transformed cells around their cycle and are killed by the cell cycle phase-specific toxic agents, in contrast to the arrested BHK cells. These findings provide a model for studies on differential killing of tumor versus normal cells in vivo.
摘要
我们在此报告，某些药物可在培养中保护叙利亚幼仓鼠肾细胞（BHK）免受致死剂阿糖胞苷、羟基脲和秋水仙酰胺的影响。多瘤病毒转化的BHK细胞（PyBHK）在相同条件下会被杀死。具有保护作用的药物包括咖啡因和链霉抗生物素蛋白A。动力学研究表明，这些药物在G1期具有特异性作用，并且它们在限制点将BHK细胞从G1期转变为G0期，这与高细胞密度或血清剥夺的作用相似。这些药物对PyBHK细胞生长的阻断效果远不如对BHK细胞，这与病毒转化细胞中限制点控制的有效性降低一致。因此，与停滞的BHK细胞不同，转化细胞在其细胞周期中循环，并被细胞周期阶段特异性毒性剂杀死。这些发现为体内肿瘤细胞与正常细胞差异杀伤的研究提供了一个模型。
相似文献
1
Selective killing of transformed baby hamster kidney (BHK) cells.选择性杀死转化的幼仓鼠肾（BHK）细胞。
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4994-8. doi: 10.1073/pnas.72.12.4994.
2
Dimethyl sulfoxide-induced reversion of several features of polyoma transformed baby hamster kidney cells (BHK-21). Alterations in growth and morphology.二甲基亚砜诱导多瘤病毒转化的幼仓鼠肾细胞（BHK - 21）的若干特征逆转。生长和形态的改变。
J Cell Biol. 1973 Apr;57(1):38-53. doi: 10.1083/jcb.57.1.38.
3
Contact behaviour and pattern formation of BHK and polyoma virus-transformed BHK fibroblasts in culture.
J Cell Sci. 1978 Oct;33:53-84. doi: 10.1242/jcs.33.1.53.
4
Transformation by polyoma virus alters expression of a cell mutation affecting cycle traverse.多瘤病毒介导的转化会改变影响细胞周期进程的细胞突变的表达。
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2540-4. doi: 10.1073/pnas.72.7.2540.
5
Modification of diamine oxidase activity in vitro by metabolites of asparagine and differences in asparagine decarboxylation in normal and virus-transformed baby hamster kidney cells.天冬酰胺代谢产物对二胺氧化酶体外活性的修饰以及正常和病毒转化的幼仓鼠肾细胞中天冬酰胺脱羧作用的差异
Biochem J. 1976 Sep 1;157(3):599-608. doi: 10.1042/bj1570599.
6
Polyoma virus transformation and two-dimensional electrophoresis patterns of baby hamster kidney cell native proteins.
Exp Cell Biol. 1984;52(3):183-8. doi: 10.1159/000163259.
7
The deformability of BHK cells and polyoma virus-transformed BHK cells in relation to locomotory behaviour.
J Cell Sci. 1980 Aug;44:187-200. doi: 10.1242/jcs.44.1.187.
8
The abnormal morphology of polyoma-transformed baby hamster kidney cells is due to a failure to respond to 70K spreading factor.多瘤病毒转化的幼仓鼠肾细胞的异常形态是由于对70K扩散因子无反应所致。
J Cell Sci. 1982 Jun;55:301-16. doi: 10.1242/jcs.55.1.301.
9
Differential cytotoxicity between transformed and normal human cells with combinations of aminonucleoside and hydroxyurea.氨基核苷与羟基脲联合使用时对人转化细胞和正常细胞的细胞毒性差异
Cancer Res. 1977 Jul;37(7 Pt 1):2126-31.
10
Comparison of N-acetylglucosaminyltransferase V activities in Rous sarcoma-transformed baby hamster kidney (RS-BHK) and BHK cells.
J Cell Biochem. 1988 Jun;37(2):225-31. doi: 10.1002/jcb.240370209.
引用本文的文献
1
Selective protection of normal cells from chemotherapy, while killing drug-resistant cancer cells.选择性保护正常细胞免受化疗的影响，同时杀死耐药性癌细胞。
Oncotarget. 2023 Mar 11;14:193-206. doi: 10.18632/oncotarget.28382.
2
Inhibition of post-transcriptional steps in ribosome biogenesis confers cytoprotection against chemotherapeutic agents in a p53-dependent manner.抑制核糖体生物发生过程中的转录后步骤以依赖 p53 的方式赋予细胞对抗化学治疗剂的细胞保护作用。
Sci Rep. 2017 Aug 22;7(1):9041. doi: 10.1038/s41598-017-09002-w.
3
Human Lung Carcinoma Reaction against Metabolic Serum Deficiency Stress.人肺癌对代谢性血清缺乏应激的反应
Iran J Pharm Res. 2016 Fall;15(4):817-823.
4
Persistence to anti-cancer treatments in the stationary to proliferating transition.从静止期到增殖期转变过程中对抗癌治疗的耐受性
Cell Cycle. 2016 Dec 16;15(24):3442-3453. doi: 10.1080/15384101.2016.1248006. Epub 2016 Nov 1.
5
New distinct compartments in the G2 phase of the cell cycle defined by the levels of γH2AX.由γH2AX水平定义的细胞周期G2期新的不同区室。
Cell Cycle. 2015;14(20):3261-9. doi: 10.1080/15384101.2015.1087617.
6
Staurosporine is chemoprotective by inducing G1 arrest in a Chk1- and pRb-dependent manner.星孢菌素通过依赖于 Chk1 和 pRb 的方式诱导 G1 期阻滞而具有化学保护作用。
Carcinogenesis. 2013 Oct;34(10):2244-52. doi: 10.1093/carcin/bgt186. Epub 2013 May 30.
7
Pharmacologic modulation of serine/threonine phosphorylation highly sensitizes PHEO in a MPC cell and mouse model to conventional chemotherapy.药物调节丝氨酸/苏氨酸磷酸化可显著提高 MPC 细胞和小鼠模型中 PHEO 对常规化疗的敏感性。
PLoS One. 2011 Feb 14;6(2):e14678. doi: 10.1371/journal.pone.0014678.
8
Impaired DNA damage response--an Achilles' heel sensitizing cancer to chemotherapy and radiotherapy.DNA 损伤反应受损——使癌症对化疗和放疗敏感的阿喀琉斯之踵。
Eur J Pharmacol. 2009 Dec 25;625(1-3):143-50. doi: 10.1016/j.ejphar.2009.05.032. Epub 2009 Oct 18.
9
The enigmatic effects of caffeine in cell cycle and cancer.咖啡因对细胞周期和癌症的神秘影响。
Cancer Lett. 2007 Mar 8;247(1):26-39. doi: 10.1016/j.canlet.2006.03.032. Epub 2006 May 18.
10
Caffeine and human DNA metabolism: the magic and the mystery.咖啡因与人类DNA代谢：神奇与奥秘
Mutat Res. 2003 Nov 27;532(1-2):85-102. doi: 10.1016/j.mrfmmm.2003.08.012.
本文引用的文献
1
Inhibition of protein synthesis in reticulocytes by antibiotics. II. The site of action of cycloheximide, streptovitacin A and pactamycin.抗生素对网织红细胞中蛋白质合成的抑制作用。II. 环己酰亚胺、链霉抗生物素A和密旋霉素的作用位点。
Biochim Biophys Acta. 1966 Apr 18;119(1):120-9.
2
Effects of caffeine on ultraviolet-irradiated mouse L cells.
Radiat Res. 1969 Jul;39(1):207-21.
3
4
Effects of post-treatment with caffeine on the sensitivity to ultraviolet light irradiation of two lines of HeLa cells.
Mutat Res. 1970 Jul;10(1):67-72. doi: 10.1016/0027-5107(70)90147-8.
5
Stimulation by serum of multiplication of stationary chicken cells.血清对静止鸡细胞增殖的刺激作用。
J Cell Physiol. 1971 Oct;78(2):161-70. doi: 10.1002/jcp.1040780202.
6
Antagonism between DNA synthesis inhibitors and protein synthesis inhibitors in mammalian cell cultures.
Cancer Res. 1974 Apr;34(4):778-82.
7
Selective inhibition of the cell cycle of cultured human diploid fibroblasts by aminonucleoside of puromycin.嘌呤霉素氨基核苷对培养的人二倍体成纤维细胞细胞周期的选择性抑制作用。
J Cell Physiol. 1973 Feb;81(1):71-83. doi: 10.1002/jcp.1040810109.
8
Control of the initiation of DNA synthesis in 3T3 cells: low-molecular weight nutrients.3T3细胞中DNA合成起始的调控：低分子量营养素
Proc Natl Acad Sci U S A. 1974 Aug;71(8):2942-5. doi: 10.1073/pnas.71.8.2942.
9
A restriction point for control of normal animal cell proliferation.正常动物细胞增殖控制的一个限制点。
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1286-90. doi: 10.1073/pnas.71.4.1286.
10
Effects of caffeine on radiation-induced phenomena associated with cell-cycle traverse of mammalian cells.咖啡因对与哺乳动物细胞周期进程相关的辐射诱导现象的影响。
Biophys J. 1974 Feb;14(2):99-118. doi: 10.1016/S0006-3495(74)70002-9.
Zotero 插件