天冬酰胺代谢产物对二胺氧化酶体外活性的修饰以及正常和病毒转化的幼仓鼠肾细胞中天冬酰胺脱羧作用的差异
Modification of diamine oxidase activity in vitro by metabolites of asparagine and differences in asparagine decarboxylation in normal and virus-transformed baby hamster kidney cells.
作者信息
Quash G, Calogero H, Fossar N, Ferdinand A, Taylor D
出版信息
Biochem J. 1976 Sep 1;157(3):599-608. doi: 10.1042/bj1570599.
Abstract
- The oxidation of putrescine in vitro by pig kidney diamine oxidase (EC 1.4.3.6) was increased in the presence of 2-oxosuccinamic acid and malonamic acid. 2. It was inhibited by 3-aminopropionamide, oxaloacetate and pyruvate. 3. 2-Oxosuccinamate was derived from asparagine in virus-transformed baby hamster kidney (BHK) cells growing in tissue culture. 4. Asparagine was decarboxylated more efficiently by transformed than by normal BHK cells. 5. In BHK cells transformed by polyoma virus (Py BHK), 2-oxosuccinamate is the most likely immediate precursor of the 14CO2 arising from [U-14C]asparagine, and there was some evidence for its formation in an asparagine-dependent clone of BHK cells before and after their transformation by hamster sarcoma virus (respectively Asn- and HSV Asn-). 6. The relationship between 2-oxosuccinamate and pyruvate and the possible roles of these two substances in controlling cellular diamine oxidase activity are discussed.
摘要
- 在2-氧代琥珀酰胺酸和丙二酰胺酸存在的情况下，猪肾二胺氧化酶（EC 1.4.3.6）在体外对腐胺的氧化作用增强。2. 它受到3-氨基丙酰胺、草酰乙酸和丙酮酸的抑制。3. 2-氧代琥珀酸酯源自组织培养中生长的病毒转化幼仓鼠肾（BHK）细胞中的天冬酰胺。4. 与正常BHK细胞相比，转化后的细胞中天冬酰胺的脱羧效率更高。5. 在多瘤病毒转化的BHK细胞（Py BHK）中，2-氧代琥珀酸酯最有可能是[U-¹⁴C]天冬酰胺产生¹⁴CO₂的直接前体，并且有证据表明在仓鼠肉瘤病毒转化前后的BHK细胞天冬酰胺依赖性克隆中（分别为Asn-和HSV Asn-）均有其形成。6. 讨论了2-氧代琥珀酸酯与丙酮酸之间的关系以及这两种物质在控制细胞二胺氧化酶活性中的可能作用。
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