Evans E W, Beach F G, Moore K M, Jackwood M W, Glisson J R, Harmon B G
Department of Veterinary Pathology, University of Georgia, Athens, USA.
Vet Microbiol. 1995 Dec;47(3-4):295-303. doi: 10.1016/0378-1135(95)00126-3.
Four avian heterophil antimicrobial cationic peptides (Chicken Heterophil Peptides 1 and 2, and Turkey Heterophil Peptides 1 and 3) were evaluated for in vitro microbicidal activity against selected avian pathogens and human pathogens which are harbored by birds. At concentrations of 16-2 micrograms/ml, all four avian peptides effected a greater than 90% reduction in the survival of Candida albicans, Salmonella enteriditis, and Campylobacter jejuni. None of the peptides, including the known antimicrobial peptide protamine (used as a positive control), were able to reduce the survival of Pasteurella multocida by 90% at the maximum peptide concentration (16 micrograms/ml) tested. At 16 micrograms/ml, the turkey peptide THP3 did not effect a 90% reduction in survival of Bordetella avium, Escherichia coli, or Salmonella typhimurium, while all of the other peptides tested were effective at this concentration or less. This peptide, THP3, does not share the same homologous amino acid sequence shared by the other three peptides. Under our experimental conditions, none of the peptides neutralized Infectious Bronchitis Virus, an enveloped coronavirus of chickens.
对四种禽类嗜异性抗菌阳离子肽（鸡嗜异性肽1和2，以及火鸡嗜异性肽1和3）进行了评估，以检测其对选定的禽类病原体和鸟类携带的人类病原体的体外杀菌活性。在浓度为16 - 2微克/毫升时，所有四种禽类肽都能使白色念珠菌、肠炎沙门氏菌和空肠弯曲菌的存活率降低90%以上。在测试的最大肽浓度（16微克/毫升）下，包括已知的抗菌肽鱼精蛋白（用作阳性对照）在内，没有一种肽能够使多杀性巴氏杆菌的存活率降低90%。在16微克/毫升时，火鸡肽THP3对禽博德特氏菌、大肠杆菌或鼠伤寒沙门氏菌的存活率降低未达到90%，而测试的所有其他肽在此浓度或更低浓度时均有效。这种肽THP3与其他三种肽不具有相同的同源氨基酸序列。在我们的实验条件下，没有一种肽能中和传染性支气管炎病毒，一种鸡的有包膜冠状病毒。
