Seifer David B, Golub Elizabeth T, Lambert-Messerlian Geralyn, Springer Gayle, Holman Susan, Moxley Michael, Cejtin Helen, Nathwani Niyati, Anastos Kathryn, Minkoff Howard, Greenblatt Ruth M
Maimonides Medical Center, Department of Obstetrics & Gynecology, Brooklyn, New York, USA.
Fertil Steril. 2007 Dec;88(6):1645-52. doi: 10.1016/j.fertnstert.2007.01.122. Epub 2007 Apr 5.
To compare Müllerian inhibiting substance (MIS) levels in serum obtained during the early follicular phase to those obtained randomly during the menstrual cycle. To determine whether HIV infection influences early follicular MIS levels, an early marker of ovarian aging.
A cross-sectional study.
Women's Interagency HIV Study, a multicenter prospective study.
PATIENT(S): Serum samples obtained from 263 (187 HIV infected and 76 uninfected) participants of the Women's Interagency HIV Study who reported menstrual bleeding during the preceding 6 months and who were not taking exogenous hormones.
INTERVENTION(S): Early follicular (cycle days 2-5) MIS samples were compared with serum samples that had been obtained without regard to menstrual cycle phase. Comparison samples were obtained within 6 weeks before or within 3 to 6 months after the early follicular samples. Early follicular FSH, E(2), inhibin B, and MIS levels were also compared between the HIV infected and uninfected women.
MAIN OUTCOME MEASURE(S): Correlation between early follicular MIS and prior and subsequent samples. Comparison of serum markers of ovarian reserve between HIV positive and negative women.
RESULT(S): The MIS values from early follicular and other random cycle phases were highly correlated with each other (r > 0.93). In multivariate analysis, increased age and FSH level and lower inhibin B levels were associated with lower MIS level; MIS values did not vary by HIV serostatus.
CONCLUSION(S): Without regard to cycle phase, MIS was similar during early follicular phase and highly correlated with early follicular FSH and inhibin B in women with and without HIV. Measurement of serum MIS offers a simplified method of determining ovarian reserve using specimens obtained without menstrual phase timing. Furthermore, using biologic measures of reproductive aging, we found no evidence that HIV infection influences ovarian aging.
比较卵泡早期血清中苗勒管抑制物质（MIS）水平与月经周期中随机采集的血清中该物质水平。确定人类免疫缺陷病毒（HIV）感染是否会影响卵泡早期MIS水平，这是卵巢衰老的一个早期标志物。
横断面研究。
妇女机构间HIV研究，一项多中心前瞻性研究。
从妇女机构间HIV研究的263名参与者（187名HIV感染者和76名未感染者）中采集血清样本，这些参与者在过去6个月内有月经出血且未服用外源性激素。
将卵泡早期（月经周期第2 - 5天）的MIS样本与不考虑月经周期阶段采集的血清样本进行比较。对照样本在卵泡早期样本采集前6周内或采集后3至6个月内获取。还比较了HIV感染和未感染女性的卵泡早期促卵泡生成素（FSH）、雌二醇（E₂）、抑制素B和MIS水平。
卵泡早期MIS与之前及后续样本之间的相关性。HIV阳性和阴性女性之间卵巢储备血清标志物的比较。
卵泡早期和其他随机周期阶段的MIS值彼此高度相关（r > 0.93）。在多变量分析中，年龄增加、FSH水平升高和抑制素B水平降低与MIS水平降低相关；MIS值不因HIV血清学状态而异。
无论周期阶段如何，卵泡早期的MIS与HIV感染与否的女性在卵泡早期的FSH和抑制素B高度相关且相似。测量血清MIS提供了一种使用无需考虑月经周期时间采集的标本确定卵巢储备的简化方法。此外，通过生殖衰老的生物学指标，我们没有发现证据表明HIV感染会影响卵巢衰老。
