Shinzato Naoya, Muramatsu Mizuho, Matsui Toru, Watanabe Yoshio
Center of Molecular Biosciences, University of the Ryukyus, Okinawa, Japan.
Biosci Biotechnol Biochem. 2007 Apr;71(4):906-15. doi: 10.1271/bbb.60540. Epub 2007 Apr 7.
We constructed a bacterial 16S rRNA gene clone library from the gut microbial community of O. formosanus and phylogenetically analyzed it in order to contribute to the evolutional study of digestive symbiosis and method development for termite control. After screening by restriction fragment length polymorphism (RFLP) analysis, 56 out of 280 clones with unique RFLP patterns were sequenced and phylogenetically analyzed. The representative phylotypes were affiliated to four phylogenetic groups, Firmicutes, the Bacteroidetes/Chlorobi group, Proteobacteria, and Actinobacteria of the domain Bacteira. No one clone affiliated with the phylum Spirochaetes was identified, in contrast to the case of wood-feeding termites. The phylogenetic analysis revealed that nearly half of the representative clones (25 phylotypes) formed monophyletic clusters with clones obtained from other termite species, especially with the sequences retrieved from fungus-growing termites. These results indicate that the presence of termite-specific bacterial lineages implies a coevolutional relationship of gut microbes and host termites.
我们从台湾乳白蚁肠道微生物群落构建了一个细菌16S rRNA基因克隆文库，并对其进行系统发育分析，以促进消化共生进化研究及白蚁防治方法的开发。经限制性片段长度多态性（RFLP）分析筛选后，对280个具有独特RFLP模式的克隆中的56个进行了测序和系统发育分析。代表性系统发育型隶属于细菌域的四个系统发育类群：厚壁菌门、拟杆菌/绿菌门、变形菌门和放线菌门。与取食木材的白蚁不同，未鉴定出任何属于螺旋体门的克隆。系统发育分析表明，近一半的代表性克隆（25个系统发育型）与从其他白蚁物种获得的克隆形成单系簇，特别是与从培菌白蚁中检索到的序列。这些结果表明，白蚁特异性细菌谱系的存在意味着肠道微生物与宿主白蚁之间存在共同进化关系。
