一种用于检测产气荚膜梭菌肠毒素抗体的酶联免疫吸附测定法。
An enzyme-linked immunosorbent assay for the detection of Clostridium perfringens enterotoxin antibody.
作者信息
Niilo L, Cho H J
出版信息
Can J Comp Med. 1984 Jan;48(1):111-2.
Abstract
An enzyme-linked immunosorbent assay (ELISA) was adapted to test serum antibody to enterotoxin of Clostridium perfringens type A. The test was evaluated using sheep, calf and guinea pig sera and compared with passive hemagglutination and immunodiffusion tests. The ELISA was found to be more sensitive than the other two tests and was completely free from nonspecific reactions. The method was considered to be technically advantageous and suitable for semiautomated procedures.
摘要
采用酶联免疫吸附测定法（ELISA）检测A型产气荚膜梭菌肠毒素的血清抗体。使用绵羊、小牛和豚鼠血清对该检测方法进行评估，并与被动血凝试验和免疫扩散试验进行比较。结果发现ELISA比其他两种检测方法更灵敏，且完全没有非特异性反应。该方法在技术上具有优势，适用于半自动操作程序。
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Can J Microbiol. 1980 Sep;26(9):1162-4. doi: 10.1139/m80-192.
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Detection of Clostridium perfringens enterotoxin in human fecal samples and anti-enterotoxin in sera.检测人粪便样本中的产气荚膜梭菌肠毒素及血清中的抗肠毒素。
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