Yoo Yung-Choon, Shin Byung-Hoon, Hong Jang-Hee, Lee Junglim, Chee Hee-Youn, Song Kyung-Sik, Lee Kyung-Bok
Department of Biochemistry, College of Medicine, Konyang University, Daejeon 302-801, Korea.
Arch Pharm Res. 2007 Mar;30(3):361-5. doi: 10.1007/BF02977619.
In this study, biologically active compounds were isolated from Protaetia brevitarsis larva (PBL) by dichloromethane extraction. The dichloromethane extract from PBL was highly cytotoxic to various cancer cells. From a silica gel column chromatograpy of this extract, we obtained four fractions (F-2, F-4, F-5 and F-7) having apoptosis-inducing activity. These fractions induced DNA ladder and caspase-3 activation during apoptosis in colon 26 tumor cells. In 1H and 13C NMR and mass spectral analysis of the fraction F-2 showing the highest apoptosis-inducing activity, we found that the fraction was composed of three free fatty acids such as palmitic acid, (Z)-9-octadecenoic acid and octadecenoic acid. These results indicate that the dichloromethane extract of PBL includes anticancer components composed of at least three fatty acids, and apoptosis-inducing activity of the extract was mediated by caspase-3 activation in tumor cells.
在本研究中，通过二氯甲烷萃取从短星翅芫菁幼虫（PBL）中分离出生物活性化合物。PBL的二氯甲烷提取物对多种癌细胞具有高度细胞毒性。通过对该提取物进行硅胶柱色谱分离，我们获得了具有凋亡诱导活性的四个组分（F-2、F-4、F-5和F-7）。这些组分在结肠26肿瘤细胞凋亡过程中诱导了DNA梯形条带的出现和半胱天冬酶-3的激活。在对显示出最高凋亡诱导活性的F-2组分进行的1H和13C核磁共振以及质谱分析中，我们发现该组分由三种游离脂肪酸组成，即棕榈酸、（Z）-9-十八碳烯酸和十八碳烯酸。这些结果表明，PBL的二氯甲烷提取物包含至少由三种脂肪酸组成的抗癌成分，并且该提取物的凋亡诱导活性是由肿瘤细胞中的半胱天冬酶-3激活介导的。
