博尔纳病病毒的X蛋白在病毒增殖周期中发挥着重要作用。
The X protein of borna disease virus serves essential functions in the viral multiplication cycle.
作者信息
Poenisch Marion, Wille Sandra, Ackermann Andreas, Staeheli Peter, Schneider Urs
机构信息
Department of Virology, University of Freiburg, Hermann-Herder-Strasse 11, D-79104 Freiburg, Germany.
出版信息
J Virol. 2007 Jul;81(13):7297-9. doi: 10.1128/JVI.02468-06. Epub 2007 Apr 11.
Abstract
The X gene of Borna disease virus (BDV) encodes a nonstructural 10-kDa protein that can interact with viral polymerase cofactor P, thus regulating polymerase activity. It remained unknown whether X is essential for virus multiplication. All our attempts to generate mutant BDV with a nonfunctional X gene proved unsuccessful. However, a mutant virus with an inactive X gene was able to replicate in Vero cells if an artificial gene cassette encoding X was inserted at a site near the 5' end of the viral genome. These results indicate that X performs essential viral functions.
摘要
博尔纳病病毒（BDV）的X基因编码一种非结构10 kDa蛋白，该蛋白可与病毒聚合酶辅因子P相互作用，从而调节聚合酶活性。X基因对于病毒增殖是否必不可少尚不清楚。我们所有构建X基因无功能的突变型BDV的尝试均未成功。然而，如果将编码X的人工基因盒插入病毒基因组5'端附近的位点，X基因失活的突变病毒能够在Vero细胞中复制。这些结果表明，X基因发挥着至关重要的病毒功能。
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本文引用的文献
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