一种用于在啮齿动物神经元中表达外源基因的博尔纳病病毒载体。
A Borna disease virus vector for expression of foreign genes in neurons of rodents.
作者信息
Schneider Urs, Ackermann Andreas, Staeheli Peter
机构信息
Department of Virology, University of Freiburg, Hermann-Herder-Strasse 11, D-79104 Freiburg, Germany.
出版信息
J Virol. 2007 Jul;81(13):7293-6. doi: 10.1128/JVI.02467-06. Epub 2007 Apr 11.
Abstract
An expression cassette for green fluorescent protein was successfully inserted at a site near the 5' end of the genome of Borna disease virus (BDV). When introduced into a mutant virus with highly active polymerase, the foreign gene was strongly expressed in neurons of infected rats. Since BDV can establish long-term persistence in the central nervous system of rodents, it may be used to engineer efficient vectors for specific delivery of foreign genes into highly differentiated neurons.
摘要
绿色荧光蛋白表达盒成功插入到博尔纳病病毒（BDV）基因组5'端附近的一个位点。当将其导入具有高活性聚合酶的突变病毒中时，外源基因在受感染大鼠的神经元中强烈表达。由于BDV可在啮齿动物的中枢神经系统中长期持续存在，它可能被用于构建高效载体，以便将外源基因特异性递送至高度分化的神经元。
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