Poenisch Marion, Unterstab Gunhild, Wolff Thorsten, Staeheli Peter, Schneider Urs
Abteilung Virologie, Institut für Medizinische Mikrobiologie & Hygiene, Universität Freiburg, D-79104 Freiburg, Germany.
Robert-Koch-Institut, Nordufer 20, D-13353 Berlin, Germany.
J Gen Virol. 2004 Jul;85(Pt 7):1895-1898. doi: 10.1099/vir.0.80002-0.
Borna disease virus polymerase activity is negatively regulated by the viral X protein. Using a virus minireplicon system it was found that all X mutants that no longer interacted with the viral P protein failed to exhibit significant inhibitory activity. The action of X could further be neutralized by expression of a P fragment that contained the X interaction domain but lacked all domains known to mediate interaction with other viral proteins. X thus appears to regulate the activity of the Borna disease virus polymerase by targeting the polymerase cofactor P.
博尔纳病病毒聚合酶活性受到病毒X蛋白的负调控。利用病毒微型复制子系统发现，所有不再与病毒P蛋白相互作用的X突变体均未表现出显著的抑制活性。通过表达一个包含X相互作用结构域但缺乏所有已知介导与其他病毒蛋白相互作用的结构域的P片段，X的作用可进一步被中和。因此，X似乎通过靶向聚合酶辅因子P来调节博尔纳病病毒聚合酶的活性。
