使用量子点的荧光相关光谱学：进展、挑战与机遇

Fluorescence correlation spectroscopy using quantum dots: advances, challenges and opportunities.

作者信息

Heuff Romey F, Swift Jody L, Cramb David T

机构信息

Department of Chemistry, University of Calgary, 2500 University Dr NW, Calgary, AB, Canada T2N 1N4.

出版信息

Phys Chem Chem Phys. 2007 Apr 28;9(16):1870-80. doi: 10.1039/b617115j. Epub 2007 Mar 2.

DOI:10.1039/b617115j

PMID:17431516

Abstract

Semiconductor nanocrystals (quantum dots) have been increasingly employed in measuring the dynamic behavior of biomacromolecules using fluorescence correlation spectroscopy. This poses a challenge, because quantum dots display their own dynamic behavior in the form of intermittent photoluminescence, also known as blinking. In this review, the manifestation of blinking in correlation spectroscopy will be explored, preceded by an examination of quantum dot blinking in general.

摘要

半导体纳米晶体（量子点）越来越多地被用于利用荧光相关光谱法测量生物大分子的动态行为。这带来了一个挑战，因为量子点以间歇性光致发光的形式表现出自身的动态行为，这种现象也被称为闪烁。在本综述中，将先对量子点闪烁进行一般性考察，然后探讨相关光谱中闪烁的表现形式。