Konishi Teruaki, Amemiya Kuniaki, Natsume Toshiyuki, Takeyasu Akihiro, Yasuda Nakahiro, Furusawa Yoshiya, Hieda Kotaro
Department of Technical Support and Development, National Institute of Radiological Sciences, 4-9-1 Anagawa, Ingeku, Chiba 263-8555, Japan.
J Radiat Res. 2007 May;48(3):255-61. doi: 10.1269/jrr.06078. Epub 2007 Apr 16.
The geometric locations of ion traversals in mammalian cells constitute important information in the study of heavy ion-induced biological effects. We employed a contact microscopy technique, which was developed for boron imaging in boron neutron capture therapy to the irradiation mammalian cells by low-energy heavy ions. This method enables the simultaneous visualization of mammalian cells as a relief on a plastic track detector, CR-39, and the etch pits which indicate the positions of ion traversals. This technique provides visual geometric information about the cells and ion traversal, without any specially designed devices or microscopes. Only common laboratory equipment, such as a conventional optical microscope, a UV lamp, and commercially available CR-39 is required. To validate this method, CHO-K1 and HeLa cells were cultured on the CR-39 surface and then irradiated with low-energy Ar and Ne ions, respectively. The positions of induced DNA double strand breaks were detected as gamma-H2AX fluorescent spots, which coincided with the positions of the etch pits in the cell relief image.
离子在哺乳动物细胞中的穿行几何位置是重离子诱导生物效应研究中的重要信息。我们采用了一种接触显微镜技术，该技术最初是为硼中子俘获治疗中的硼成像而开发的，用于低能重离子辐照哺乳动物细胞。这种方法能够同时将哺乳动物细胞以浮雕形式可视化于塑料径迹探测器CR-39上，并能看到指示离子穿行位置的蚀刻坑。该技术无需任何专门设计的设备或显微镜，就能提供有关细胞和离子穿行的视觉几何信息。仅需常规实验室设备，如传统光学显微镜、紫外灯和市售的CR-39即可。为验证该方法，将CHO-K1和HeLa细胞分别培养在CR-39表面，然后用低能氩离子和氖离子进行辐照。诱导的DNA双链断裂位置被检测为γ-H2AX荧光斑点，其与细胞浮雕图像中蚀刻坑的位置相符。
