Nylander G, Berg K
Institute of Medical Genetics, University of Oslo, Norway.
Int Arch Occup Environ Health. 1991;63(4):229-32. doi: 10.1007/BF00386370.
Urine from 32 road tanker drivers handling petrol, diesel, paraffin and white spirit was tested in the Salmonella/microsome mutagenicity system using tester strains TA98 anbd TA100. Urine samples were collected before and after tanking and were concentrated 250-fold on XAD-2 columns before testing. Controls were 33 office employees from the same plant. No effect on urinary mutagenicity of occupational exposure to petroleum products was found. Significant differences in mutagenic effect were demonstrated between smokers and non-smokers. Methodological experiments demonstrated a dose/response effect of smoking on urinary mutagenicity. A modified test using a preincubation period and an increased concentration of bacteria was more sensitive than the standard Ames test to mutagens from tobacco smoking.
对32名装卸汽油、柴油、石蜡和白酒精的油罐车司机的尿液，在沙门氏菌/微粒体诱变性系统中使用测试菌株TA98和TA100进行了检测。尿液样本在装罐前后采集，并在检测前在XAD - 2柱上浓缩250倍。对照组是来自同一工厂的33名办公室职员。未发现职业性接触石油产品对尿液诱变性有影响。吸烟者与不吸烟者之间在诱变效应上显示出显著差异。方法学实验证明吸烟对尿液诱变性有剂量/反应效应。一种使用预温育期和增加细菌浓度的改良试验，比标准的艾姆斯试验对吸烟产生的诱变剂更敏感。
