Poly Frédéric, Read Timothy, Tribble David R, Baqar Shahida, Lorenzo Maria, Guerry Patricia
Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20906, USA.
Infect Immun. 2007 Jul;75(7):3425-33. doi: 10.1128/IAI.00050-07. Epub 2007 Apr 16.
Campylobacter jejuni CG8486, which belongs to the HS4 complex, was isolated from a patient with inflammatory diarrhea in Thailand. This strain caused a diarrheal disease in ferrets comparable to that caused by C. jejuni strain 81-176, but it was much less invasive for epithelial cells in vitro than 81-176. Complete genome sequencing of CG8486 revealed a 1.65-Mb genome that was very similar to the other two published genomes of clinical isolates of C. jejuni, the genomes of 81-176 and NCTC 11168, with a limited number of CG8486-specific genes mapping outside the hypervariable carbohydrate biosynthesis loci. These data suggest that the genes required for induction of inflammatory diarrhea are among the genes shared by CG8486 and 81-176 but that either major changes in the carbohydrate loci and/or more subtle changes in other genes may modulate virulence.
空肠弯曲菌CG8486属于HS4复合体，是从泰国一名患有炎性腹泻的患者体内分离出来的。该菌株在雪貂中引起的腹泻病与空肠弯曲菌81-176菌株所致的腹泻病相当，但在体外对上皮细胞的侵袭性比81-176菌株小得多。CG8486的全基因组测序显示其基因组大小为1.65 Mb，与另外两个已发表的空肠弯曲菌临床分离株基因组（81-176和NCTC 11168的基因组）非常相似，只有少数CG8486特异性基因定位于高变碳水化合物生物合成位点之外。这些数据表明，诱导炎性腹泻所需的基因存在于CG8486和81-176共有的基因之中，但碳水化合物位点的重大变化和/或其他基因的更细微变化可能会调节毒力。
