大肠杆菌中编码乙醇脱氢酶的adhE基因的调控。
Regulation of the adhE gene, which encodes ethanol dehydrogenase in Escherichia coli.
作者信息
Chen Y M, Lin E C
机构信息
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
出版信息
J Bacteriol. 1991 Dec;173(24):8009-13. doi: 10.1128/jb.173.24.8009-8013.1991.
Abstract
The respiratory control of adhE, which encodes ethanol (alcohol) dehydrogenase in Escherichia coli, was examined at the transcriptional level by using various phi (adhE'-lacZ) adhE+ merodiploid strains. Expression of the adhE'-lacZ operon fusion was increased about eightfold by anaerobic growth. Under anaerobic growth conditions, provision of nitrate lowered the level of expression. Nitrate repression was more severe than aerobic repression. From analyses of various mutants with mutations related to nitrate reduction, nitrate repression appears to result from two effects. (i) When nitrate was present, NarL, the positive regulator of the nar operon, exerted a direct repression on adhE expression, which was demonstrable even aerobically. (ii) The chemical reduction of nitrate exerted an indirect effect by altering the cellular redox potential.
摘要
通过使用各种φ(adhE'-lacZ) adhE+部分二倍体菌株，在转录水平上研究了大肠杆菌中编码乙醇脱氢酶的adhE的呼吸控制。厌氧生长使adhE'-lacZ操纵子融合体的表达增加了约八倍。在厌氧生长条件下，提供硝酸盐会降低表达水平。硝酸盐阻遏比有氧阻遏更严重。通过对与硝酸盐还原相关的各种突变体的分析，硝酸盐阻遏似乎是由两种效应导致的。(i) 当存在硝酸盐时，nar操纵子的正调控因子NarL对adhE表达施加直接阻遏，即使在有氧条件下也能观察到。(ii) 硝酸盐的化学还原通过改变细胞氧化还原电位产生间接效应。
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