Lusic Hrvoje, Young Douglas D, Lively Mark O, Deiters Alexander
Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
Org Lett. 2007 May 10;9(10):1903-6. doi: 10.1021/ol070455u. Epub 2007 Apr 21.
A new photocaged nucleoside was synthesized and incorporated into DNA with the use of standard synthesis conditions. This approach enabled the disruption of specific H-bonds and allowed for the analysis of their contribution to the activity of a DNAzyme. Brief irradiation with nonphotodamaging UV light led to rapid decaging and almost quantitative restoration of DNAzyme activity. The developed strategy has the potential to find widespread application in the light-induced regulation of oligonucleotide function.
合成了一种新的光笼核苷，并使用标准合成条件将其掺入DNA中。这种方法能够破坏特定的氢键，并允许分析它们对脱氧核酶活性的贡献。用无光损伤的紫外光进行短暂照射会导致快速脱笼，并使脱氧核酶活性几乎定量恢复。所开发的策略有可能在光诱导的寡核苷酸功能调控中得到广泛应用。
