光化学DNA激活
Photochemical DNA activation.
作者信息
Lusic Hrvoje, Young Douglas D, Lively Mark O, Deiters Alexander
机构信息
Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.
出版信息
Org Lett. 2007 May 10;9(10):1903-6. doi: 10.1021/ol070455u. Epub 2007 Apr 21.
Abstract
A new photocaged nucleoside was synthesized and incorporated into DNA with the use of standard synthesis conditions. This approach enabled the disruption of specific H-bonds and allowed for the analysis of their contribution to the activity of a DNAzyme. Brief irradiation with nonphotodamaging UV light led to rapid decaging and almost quantitative restoration of DNAzyme activity. The developed strategy has the potential to find widespread application in the light-induced regulation of oligonucleotide function.
摘要
合成了一种新的光笼核苷，并使用标准合成条件将其掺入DNA中。这种方法能够破坏特定的氢键，并允许分析它们对脱氧核酶活性的贡献。用无光损伤的紫外光进行短暂照射会导致快速脱笼，并使脱氧核酶活性几乎定量恢复。所开发的策略有可能在光诱导的寡核苷酸功能调控中得到广泛应用。
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