Moshynskyy Igor, Viswanathan Sathiyanarayanan, Vasilenko Natalia, Lobanov Vladislav, Petric Martin, Babiuk Lorne A, Zakhartchouk Alexander N
Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada.
Virus Res. 2007 Jul;127(1):116-21. doi: 10.1016/j.virusres.2007.03.011. Epub 2007 Apr 19.
Open reading frame 9b (ORF 9b) encodes a 98 amino acid group-specific protein of severe acute respiratory syndrome (SARS) coronavirus (CoV). It has no homology with known proteins and its function in SARS CoV replication has not been determined. The N-terminal part of the 9b protein was used to raise polyclonal antibodies in rabbits, and these antibodies could detect 9b protein in infected cells. We analyzed the sub-cellular localization of recombinant 9b protein using fluorescence microscopy of live transfected cells and indirect immunofluorescence of transfected fixed cells. Our findings indicate that the 9b protein is exported outside of a cell nucleus and localizes to the endoplasmic reticulum. Our data also suggest that the 46-LRLGSQLSL-54 amino acid sequence of 9b functions as a nuclear export signal (NES).
开放阅读框9b（ORF 9b）编码一种严重急性呼吸综合征（SARS）冠状病毒（CoV）的98个氨基酸的群特异性蛋白。它与已知蛋白无同源性，其在SARS冠状病毒复制中的功能尚未确定。利用9b蛋白的N端部分在兔体内制备多克隆抗体，这些抗体可在感染细胞中检测到9b蛋白。我们通过对活转染细胞的荧光显微镜观察和对转染固定细胞的间接免疫荧光分析，对重组9b蛋白的亚细胞定位进行了分析。我们的研究结果表明，9b蛋白从细胞核输出并定位于内质网。我们的数据还表明，9b蛋白的46-LRLGSQLSL-54氨基酸序列作为核输出信号（NES）发挥作用。
