MEGA（美利奴羊×加鲁特羊）绵羊BMPR1B基因中携带Booroola（Fec）等位基因的检测及其与生长性状的关联
Detection of carrier Booroola (Fec) allele in BMPR1B gene of MEGA (Merino × Garut) sheep and its association with growth traits.
作者信息
Margawati Endang Tri, Putra Widya Pintaka Bayu, Rizki Muhammad, Soetrisno Edi, Raadsma Herman Willem
机构信息
Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Bogor, 16911, Indonesia.
Department of Animal Science, Faculty of Agriculture, University of Bengkulu, Bengkulu, 38371, Indonesia.
出版信息
J Genet Eng Biotechnol. 2023 Feb 15;21(1):19. doi: 10.1186/s43141-023-00475-z.
BACKGROUND
Bone morphogenetic protein receptor 1B (BMPR1B) gene is one of candidate genes for reproductive and growth traits in sheep. The present study was aimed to detect the Booroola (Fec) allele in BMPR1B gene and its association with growth traits in MEGA (Merino × Garut) sheep. A total of 82DNA samples collected from individual lamb (mixed-sex) blood were genotyped for allelic polymorphism using a PCR-RFLP method.
RESULTS
The PCR analysis in BMPR1B gene resulted the amplicons with size of140 bp. The RFLP analysis with AvaII restriction enzymeresultedtwo allelic types of wildtype (A/Fec) and mutant or Booroola (G/Fec) with frequency of 0.89 and 0.11, respectively. However, the genetic diversity in BMPR1B/AvaII gene of animal studies was categorized tolow category (PIC = 0.18)and under in a genetic equilibrium (χ = 1.25).
CONCLUSIONS
Itshowed us that carrying Fec allele in the heterozygous sheep were not associated with growth traits in MEGA sheep.
背景
骨形态发生蛋白受体1B（BMPR1B）基因是绵羊繁殖和生长性状的候选基因之一。本研究旨在检测BMPR1B基因中的Booroola（Fec）等位基因及其与MEGA（美利奴×加鲁特）绵羊生长性状的关联。使用PCR-RFLP方法对从个体羔羊（混合性别）血液中采集的82份DNA样本进行等位基因多态性基因分型。
结果
BMPR1B基因的PCR分析得到了大小为140 bp的扩增子。用AvaII限制性内切酶进行的RFLP分析产生了两种等位基因类型，即野生型（A/Fec）和突变型或Booroola（G/Fec），频率分别为0.89和0.11。然而，动物研究中BMPR1B/AvaII基因的遗传多样性被归类为低类别（PIC = 0.18）且处于遗传平衡状态（χ = 1.25）。
结论
这表明杂合绵羊携带Fec等位基因与MEGA绵羊的生长性状无关。
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