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锡林郭勒地区绵羊品种肌肉中基因型的鉴定及建立用于区分等位基因的实时荧光定量PCR技术。

Identifying genotypes in the muscle from sheep breeds indigenous to Xilingol, and establishment of a Man real-time PCR technique to distinguish alleles.

作者信息

Guo Liang, Li Chun-Dong, Liu Guo-Qiang, Luo Jian-Xing, Xu Wei-Liang, Guo Yuan-Sheng

机构信息

Xilin Gol Food Testing and Risk Assessment Center Xilingol Vocational College Xilin Gol Institute of Bioengineering Xilinhot China.

出版信息

Food Sci Nutr. 2022 Mar 30;10(7):2470-2475. doi: 10.1002/fsn3.2853. eCollection 2022 Jul.

DOI:10.1002/fsn3.2853
PMID:35844925
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9281931/
Abstract

The muscle from Xilingol indigenous sheep breeds are famous in China, and the genotype in this population remains uncharacterized. In this study, SNPs in the locus were investigated by pyrosequencing, and an optimized PCR-RFLP technique was generated to identify SNPs. In addition, an efficient technique for high-throughput identification of SNPs in was optimized using Man real-time PCR and breed-conservative primers and SNP-specific probes. By genotyping the locus in the muscle of Xilingol indigenous sheep breeds using a novel Man real-time PCR assay, our study has generated the groundwork for the authentication of Xilingol mutton based on the specific gene and the prolificacy-oriented breeding of Xilingol sheep using marker-assisted selection strategies in the future.

摘要

锡林郭勒本土绵羊品种的肌肉在中国很有名，且该群体的基因型仍未得到表征。在本研究中，通过焦磷酸测序法研究了该位点的单核苷酸多态性（SNPs），并建立了一种优化的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术来鉴定SNPs。此外，利用TaMan实时聚合酶链反应、品种保守引物和单核苷酸多态性特异性探针，优化了一种高效的高通量鉴定SNPs的技术。通过使用一种新型的TaMan实时聚合酶链反应分析法对锡林郭勒本土绵羊品种肌肉中的该位点进行基因分型，我们的研究为未来基于特定基因对锡林郭勒羊肉进行鉴定以及利用标记辅助选择策略对锡林郭勒绵羊进行多产性定向育种奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3af1/9281931/4384a240d035/FSN3-10-2470-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3af1/9281931/717b85f12d24/FSN3-10-2470-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3af1/9281931/9a298d39225f/FSN3-10-2470-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3af1/9281931/4384a240d035/FSN3-10-2470-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3af1/9281931/717b85f12d24/FSN3-10-2470-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3af1/9281931/9a298d39225f/FSN3-10-2470-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3af1/9281931/4384a240d035/FSN3-10-2470-g004.jpg

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2
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Anim Reprod Sci. 2018 Feb;189:19-29. doi: 10.1016/j.anireprosci.2017.12.003. Epub 2017 Dec 16.
3
Genetic screening of FecB, FecX and FecX mutations and their linkage with litter size in Barki and Rahmani sheep breeds.
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Reprod Domest Anim. 2017 Dec;52(6):1133-1137. doi: 10.1111/rda.13002. Epub 2017 Jun 5.
4
Increasing litter size in a sheep breed by marker-assisted selection of BMPR1B A746G mutation.通过对骨形态发生蛋白受体1B（BMPR1B）A746G突变进行标记辅助选择来增加绵羊品种的产羔数。
J Genet. 2015 Mar;94(1):139-42. doi: 10.1007/s12041-015-0470-8.
5
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