Maslyukov P M, Nozdrachev A D, Timmermans J-P
Department of Normal Physiology, Yaroslavl State Medical Academy, 5 Revolyutsionnaya Street, 150000 Yaroslavl, Russia.
Neurosci Behav Physiol. 2007 May;37(4):349-53. doi: 10.1007/s11055-007-0020-9.
The neurotransmitter composition of neurons in the stellate ganglion of rats of different ages (neonatal, 10, 20, 30, and 60 days) was studied by an immunocytochemical method using double labeling. Most neurons in rat pups of all age groups contained tyrosine hydroxylase. Most choline acetyltransferase-positive neurocytes in neonatal and 10-day-old rat pups were also tyrosine hydroxylase-positive. Only occasional cells in 30-and 60-day rat pups contained both of these enzymes. There were increases in the proportions of cells containing tyrosine hydroxylase and neuropeptide Y from birth to all time points of the study. In addition, there was a decrease in the proportion of somatostatin-positive neurons. The proportions of VIP-positive cells and choline acetyltransferase-containing neurons increased to age 10 days and then decreased. Somatostatin-positive neurons in all rat pups were small cells, while those containing choline acetyltransferase were large. Maturation of the neurotransmitter set in the rat stellate ganglion was complete by the end of the second month of life.
采用双重标记免疫细胞化学方法，研究了不同年龄（新生、10日龄、20日龄、30日龄和60日龄）大鼠星状神经节中神经元的神经递质组成。所有年龄组的幼鼠中，大多数神经元都含有酪氨酸羟化酶。新生和10日龄幼鼠中，大多数胆碱乙酰转移酶阳性神经细胞也是酪氨酸羟化酶阳性。在30日龄和60日龄的大鼠幼崽中，只有偶尔的细胞同时含有这两种酶。从出生到研究的所有时间点，含有酪氨酸羟化酶和神经肽Y的细胞比例都有所增加。此外，生长抑素阳性神经元的比例有所下降。血管活性肠肽阳性细胞和含胆碱乙酰转移酶神经元的比例在10日龄时增加，然后下降。所有大鼠幼崽中，生长抑素阳性神经元都是小细胞，而含胆碱乙酰转移酶的神经元是大细胞。大鼠星状神经节中神经递质组合的成熟在出生后第二个月末完成。
