Wu Trang-Tiau, Hsieh Yi-Hsien, Wu Cheng-Chung, Hsieh Yih-Shou, Huang Chih-Yang, Liu Jer-Yuh
Department of Surgery, School of Medicine, Medical College, Chung Shan Medical University, Taichung, Taiwan.
Clin Chim Acta. 2007 Jul;382(1-2):54-8. doi: 10.1016/j.cca.2007.03.018. Epub 2007 Mar 28.
Members of the protein kinase C (PKC) isoenzyme family play a central role in the tumorigenesis of several tissues. However, little is known about subtype specific intracellular expression of PKC in human hepatocellular carcinomas.
We investigated PKC isoforms mRNA expression in 42 HCC specimens using reverse transcription polymerase chain reaction analysis, and the correlation between PKC isoforms expression and clinicopathologic parameters.
We found that PKCalpha, PKCdelta and PKCiota mRNA were significantly increased in HCCs as compared to the corresponding non-cancerous liver tissues. PKCalpha expression also significantly correlated with tumor size (P<0.05) and TNM stage (P<0.05), but PKCdelta and PKCiota did not. The log-rank analysis revealed that patients with higher PKCalpha mRNA expression in the HCC tissues had significantly shorter survival rate than patients with lower PKCalpha mRNA expression (P<0.01).
Our results suggested that the PKCalpha may be a prognostic factor for the survival of patients with HCC.
蛋白激酶C（PKC）同工酶家族成员在多种组织的肿瘤发生中起核心作用。然而，关于PKC在人类肝细胞癌中的亚型特异性细胞内表达情况知之甚少。
我们采用逆转录聚合酶链反应分析方法，研究了42例肝癌标本中PKC亚型的mRNA表达情况，以及PKC亚型表达与临床病理参数之间的相关性。
我们发现，与相应的非癌肝组织相比，肝癌组织中PKCα、PKCδ和PKCι的mRNA显著增加。PKCα的表达也与肿瘤大小（P<0.05）和TNM分期（P<0.05）显著相关，但PKCδ和PKCι与肿瘤大小和TNM分期无关。对数秩分析显示，肝癌组织中PKCα mRNA表达较高的患者生存率显著低于PKCα mRNA表达较低的患者（P<0.01）。
我们的结果表明，PKCα可能是肝癌患者生存的一个预后因素。
