Leblanc Danielle, Ward Pierre, Gagné Marie-Josée, Poitras Elyse, Müller Peter, Trottier Yvon-Louis, Simard Carole, Houde Alain
Agriculture and Agri-Food Canada, Food Research and Development Centre, 3600 Casavant Blvd. West, Saint-Hyacinthe, Quebec, Canada J2S 8E3.
Int J Food Microbiol. 2007 Jun 30;117(2):160-6. doi: 10.1016/j.ijfoodmicro.2007.03.008. Epub 2007 Mar 30.
Hepatitis E virus has recently been recognized as having zoonotic potential and could be transmitted from pig to human. Pigs are identified as a potential animal reservoir and HEV is highly prevalent in the swine population around the world. In this study, the presence of HEV was investigated in 51 subjects reared on a simulated commercial farm setting from the age of 2 weeks up to slaughter. Samples were collected on four occasions: at 2, 8, and 18 weeks and between 22-29 weeks of age. Anti-HEV IgG in plasma samples, presence of HEV RNA in plasma samples and feces were monitored. At 2 weeks of age, HEV RNA was detected in feces of 6 subjects (11.8%) but not in their plasma. At 8 weeks, HEV was detected in feces of 27 subjects (52.9%) and in plasma of one subject. At 18 weeks, HEV was detected in feces of 44 subjects (86.2%) and in plasma of 24 subjects (47.1%). At slaughter time (22-29 weeks), HEV was present in plasma of 6 subjects (11.8%) and in stools of 21 subjects (41.2%). Spread of the virus inside the population was evaluated by comparison of means (paired t-test, P<0.05) of anti-HEV IgG ELISA results from the 4 bleedings. Significant differences were noted between the results of populations at 8 and 18 weeks and also between 18 and 22 to 29 weeks indicating an immune response to the virus. Based on the comparison of a 304 nucleotides sequence of the 5' ORF 2 gene, all amplified fragments clustered in genotype 3a.
戊型肝炎病毒最近被认为具有人畜共患病潜力，可从猪传播给人类。猪被确定为潜在的动物宿主，戊型肝炎病毒在全球猪群中高度流行。在本研究中，对51名在模拟商业农场环境中饲养的受试者进行了调查，这些受试者从2周龄直至屠宰。在四个时间点采集样本：2周、8周、18周以及22 - 29周龄之间。监测血浆样本中的抗戊型肝炎病毒IgG、血浆样本和粪便中戊型肝炎病毒RNA的存在情况。在2周龄时，6名受试者（11.8%）的粪便中检测到戊型肝炎病毒RNA，但血浆中未检测到。在8周时，27名受试者（52.9%）的粪便中检测到戊型肝炎病毒，一名受试者的血浆中检测到该病毒。在18周时，44名受试者（86.2%）的粪便中检测到戊型肝炎病毒，24名受试者（47.1%）的血浆中检测到该病毒。在屠宰时（22 - 29周），6名受试者（11.8%）的血浆中存在戊型肝炎病毒，21名受试者（41.2%）的粪便中存在该病毒。通过比较4次采血的抗戊型肝炎病毒IgG ELISA结果的均值（配对t检验，P<0.05）来评估病毒在群体中的传播情况。在8周和18周的群体结果之间以及18周和22至29周的群体结果之间发现了显著差异，表明对该病毒有免疫反应。基于5' ORF 2基因304个核苷酸序列的比较，所有扩增片段均聚类于3a基因型。
