Kang Bo Kyu, Song Dae Sub, Jung Kwon Il, Lee Chul Seung, Park Sung Jun, Oh Jin Sik, An Dong Jun, Yang Jeong Sun, Moon Hyoung Joon, Lee Sang Sun, Yoon Young Dhuk, Park Bong Kyun
Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
J Vet Diagn Invest. 2007 Jan;19(1):78-83. doi: 10.1177/104063870701900112.
Canine rotavirus was isolated from feces of a Korean Jindo dog with mild diarrhea, and the isolate was genetically characterized. Rotaviral antigen was detected in the feces using a commercial rotavirus antigen detection kit and cytopathic effects were observed in a cell line inoculated with the feces. The virus isolate (GC/KS05) was identified as subtype G3P[3] using reverse transcription polymerase chain reaction (RT-PCR). The strain displayed 98% and 90% identity with the VP7 genes of a canine rotavirus isolate (RV52/96) from Italy and the simian rotavirus strain (RRV) respectively. However, the GC/KS05 isolate exhibited only 83% and 82% identity, respectively, with the G3 serotype canine strains, RV198/95 and K9. Phylogenetic analysis of the VP7 and VP4 genes of GC/KS05 strain led to the classification of VP7 in a different cluster than other canine rotavirus VP7 genes, and VP4 within the cluster of canine rotavirus VP4 genes. The Korean isolate was thus more closely related to the RV52/96 isolate than the other isolates for which sequence data is available. Detailed analysis of the VP7 region revealed 6 amino acid variations between the new isolate and RV52/96. After 5 passages in cell culture, the GC/KS05 strain remained pathogenic for young pups, in which inoculation resulted in diarrhea and virus shedding in the feces.
从一只患有轻度腹泻的韩国珍岛犬粪便中分离出犬轮状病毒，并对该分离株进行了基因特征分析。使用商业轮状病毒抗原检测试剂盒在粪便中检测到轮状病毒抗原，并在接种粪便的细胞系中观察到细胞病变效应。使用逆转录聚合酶链反应（RT-PCR）将病毒分离株（GC/KS05）鉴定为G3P[3]亚型。该毒株与来自意大利的犬轮状病毒分离株（RV52/96）和猿猴轮状病毒株（RRV）的VP7基因分别具有98%和90%的同源性。然而，GC/KS05分离株与G3血清型犬毒株RV198/95和K9的同源性分别仅为83%和82%。对GC/KS05毒株的VP7和VP4基因进行系统发育分析，结果显示VP7与其他犬轮状病毒VP7基因分属于不同的簇，而VP4则位于犬轮状病毒VP4基因簇内。因此，与其他有序列数据的分离株相比，该韩国分离株与RV52/96分离株的关系更为密切。对VP7区域的详细分析显示，新分离株与RV52/96之间存在6个氨基酸差异。在细胞培养中传代5次后，GC/KS05毒株对幼犬仍具有致病性，接种后会导致腹泻和粪便中病毒排出。
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