意大利两株G3P5A[3]犬轮状病毒毒株的分离与基因特征分析
Isolation and genetic characterization of two G3P5A[3] canine rotavirus strains in Italy.
作者信息
Martella V, Pratelli A, Elia G, Decaro N, Tempesta M, Buonavoglia C
机构信息
Faculty of Veterinary Medicine, Department of Animal Health and Wellbeing, University of Bari, Bari, Italy.
出版信息
J Virol Methods. 2001 Jul;96(1):43-9. doi: 10.1016/s0166-0934(01)00312-3.
Two strains of canine rotavirus were isolated from pups with clinical signs of gastroenteritis. Both strains were identified by polymerase chain reaction (PCR) as G3P5A[3], although restriction endonuclease analysis of the PCR amplicons revealed a genetic difference between the two isolates in the VP7 gene. The isolation in Italy of canine rotaviruses displaying the same VP7 and VP4 specificities as in the USA and in Japan, suggests that the G3 and P5A[3] types are highly conserved among canine rotavirus strains.
从患有肠胃炎临床症状的幼犬中分离出两株犬轮状病毒。尽管对聚合酶链反应（PCR）扩增产物进行限制性内切酶分析显示，两株分离株在VP7基因上存在遗传差异，但通过PCR均鉴定这两株病毒为G3P5A[3]。在意大利分离出的犬轮状病毒与美国和日本的病毒具有相同的VP7和VP4特异性，这表明G3和P5A[3]型在犬轮状病毒株中高度保守。
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