Kalbacova Marie, Roessler Sophie, Hempel Ute, Tsaryk Roman, Peters Kirsten, Scharnweber Dieter, Kirkpatrick James C, Dieter Peter
Institute of Physiological Chemistry, Medical Faculty of Carl Gustav Carus, Dresden University of Technology, Fiedlerstr. 42, 01307 Dresden, Germany.
Biomaterials. 2007 Aug;28(22):3263-72. doi: 10.1016/j.biomaterials.2007.02.026. Epub 2007 Mar 6.
Nowadays aseptic loosening is the most common cause of orthopaedic implant failure. Some of its reasons have already been described up to now; however, others remain still hypothetical. Besides the inflammatory response to wear particles originating at different sources, the role of reactive oxygen species as products of cellular reactions and/or as a result of the process of corrosion of an implant leading to implant failure has recently been discussed too. In the present study, we used a galvanostatic polarization to simulate the cathodic partial reaction of the corrosion process at a titanium alloy surface. With respect to cells occurring at the interface of a metal implant, the behaviour of osteoblasts and monocytes/macrophages was investigated. It has been found that cathodic polarization of Ti6Al4V induces an increase in the level of intracellular reactive oxygen species as well as suppressing the metabolic activity of cells in a dose-dependent manner. This is in agreement with the results obtained with cells after external addition of hydrogen peroxide as another kind of oxidative stress. In both approaches, monocytes/macrophages show a higher tolerance to oxidative stress than osteoblasts. It could be concluded that the electrochemical setup developed induced intracellular changes occurring during oxidative stress and it could be used for future detailed analysis of the consequences of corrosion processes for cellular reactions.
如今，无菌性松动是骨科植入物失败最常见的原因。到目前为止，其部分原因已被阐明；然而，其他一些原因仍只是假设。除了对源自不同来源的磨损颗粒的炎症反应外，活性氧作为细胞反应产物和/或植入物腐蚀过程导致植入物失败的结果所起的作用最近也受到了讨论。在本研究中，我们使用恒电流极化来模拟钛合金表面腐蚀过程的阴极部分反应。针对金属植入物界面处存在的细胞，研究了成骨细胞和单核细胞/巨噬细胞的行为。已发现Ti6Al4V的阴极极化会导致细胞内活性氧水平升高，并以剂量依赖的方式抑制细胞的代谢活性。这与在细胞外添加过氧化氢作为另一种氧化应激后获得的结果一致。在这两种方法中，单核细胞/巨噬细胞对氧化应激的耐受性都高于成骨细胞。可以得出结论，所开发的电化学装置诱导了氧化应激期间发生的细胞内变化，并且可用于未来对腐蚀过程对细胞反应影响的详细分析。
