Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, Henan, China.
Henan Key Laboratory of Farm Animal Breeding and Nutritional Regulation, Zhengzhou, 450002, Henan, China.
BMC Vet Res. 2019 Sep 2;15(1):311. doi: 10.1186/s12917-019-2060-0.
Norovirus (NoV) is recognized as a highly contagious enteric pathogen of mammals, and bovine norovirus (BNoV) is associated with calf diarrhoea and has caused great economic losses in the cattle industry.
Here, we describe a case of emerging calf diarrhoea on a cattle farm in Henan Province, Central China. BNoV was the only enteric pathogen detected in outbreaks according to tests for enteric viruses, bacteria and parasites. The complete genome of the newly identified strain CH-HNSC-2018 was successfully sequenced and found to be 7342 nucleotides in length. Sequence and phylogenetic analyses revealed that CH-HNSC-2018 belongs to GIII.2 BNoV. Further analysis of the major capsid protein demonstrated that it is separated by specific genetic distances from previous BNoV strains identified in China and has 4 new amino acid (aa) mutations, 134A, 327 T, 380 L and 423A, in the VP1 protein and 11 aa substitutions in the hypervariable P2 subdomain, suggesting that the BNoV strains circulating in China are diverse.
This is the first detection of GIII.2 BNoV in the VP1 region in China. This report should form a basis for further molecular studies on NoV and bovine enteric viruses in China.
诺如病毒（NoV）被认为是一种高度传染性的哺乳动物肠道病原体，牛诺如病毒（BNoV）与犊牛腹泻有关，并在牛业造成了巨大的经济损失。
本研究描述了中国中部河南省一个奶牛场爆发的新型犊牛腹泻病例。根据肠道病毒、细菌和寄生虫检测，BNoV 是唯一检测到的肠道病原体。新鉴定的 CH-HNSC-2018 株的全基因组成功测序，长度为 7342 个核苷酸。序列和系统发育分析表明，CH-HNSC-2018 属于 GIII.2 型 BNoV。对主要衣壳蛋白的进一步分析表明，它与中国以前鉴定的 BNoV 株有特定的遗传距离，VP1 蛋白中有 4 个新的氨基酸（aa）突变 134A、327T、380L 和 423A，P2 超变区有 11 个 aa 取代，表明中国流行的 BNoV 株具有多样性。
这是中国首次在 VP1 区检测到 GIII.2 BNoV。本报告为进一步开展中国诺如病毒和牛肠道病毒的分子研究奠定了基础。
