Adamis Z, Krass B K
National Institute of Occupational Health, Budapest, Hungary.
Ann Occup Hyg. 1991 Oct;35(5):469-83. doi: 10.1093/annhyg/35.5.469.
The effect of a number of ceramic raw materials and airborne samples in workplaces has been investigated in vitro [erythrocyte haemolysis, macrophage TTC (2,3,5-triphenyl-tetrazolium chloride) reduction and LDH (lactate dehydrogenase) activity] and in vivo (protein, LDH and phospholipid in cell-free bronchopulmonary lavage). In the in vitro experiments described we were able to distinguish between the dusts causing different types of reaction in the lung. Dusts classified as 'quartz type' in vitro were also cytotoxic in vivo. Dusts have also been divided into three groups according to their degree of hazardous nature, taking into consideration the time before an inflammatory reaction is detected and the degree of inflammatory response as characterized by biochemical parameters. One feature of the test systems which is of practical significance is that in vitro experiments require only 15 mg of dust, and in vivo experiments only 100 mg.
已经在体外[红细胞溶血、巨噬细胞TTC（2,3,5-三苯基氯化四氮唑）还原和LDH（乳酸脱氢酶）活性]和体内[无细胞支气管肺泡灌洗中的蛋白质、LDH和磷脂]研究了多种陶瓷原料和工作场所空气样本的影响。在所描述的体外实验中，我们能够区分在肺部引起不同类型反应的粉尘。在体外分类为“石英型”的粉尘在体内也具有细胞毒性。考虑到检测到炎症反应之前的时间以及以生化参数为特征的炎症反应程度，粉尘还根据其危害性质的程度分为三组。测试系统的一个具有实际意义的特点是，体外实验仅需15毫克粉尘，体内实验仅需100毫克粉尘。
