Adamis Z, Timár M, Köfler L, Tátrai E, Ungváry G
Environ Res. 1986 Oct;41(1):319-26. doi: 10.1016/s0013-9351(86)80193-1.
The biological effects of ore dusts were examined on peritoneal macrophages in vitro and on rat lung in vivo. The methylene blue adsorption of the dust samples, cytotoxicity, and phospholipid and hydroxyproline content of the lung were determined. It was realized that the examined ore samples had cytotoxic effects and from the in vitro results one can get information about the type of damage to be expected in the lung tissue. It is supposed that the examined ore dusts are less dangerous and slower acting than quartz, but they cause progressive fibrosis. The in vivo biochemical experiments do not give information about the type of damage to be expected in lung tissue; and the data on ore, bentonite, and coal do not differ from each other. To determine the typical biochemical changes caused by quartz, we obtain the most valuable information by determination of lung weight and phospholipid, phosphatidylcholine, and hydroxyproline content.
研究了矿石粉尘对体外培养的腹腔巨噬细胞和体内大鼠肺的生物学效应。测定了粉尘样品的亚甲蓝吸附量、细胞毒性以及肺组织的磷脂和羟脯氨酸含量。结果发现，所检测的矿石样品具有细胞毒性，从体外实验结果可以了解到肺组织可能受到的损伤类型。据推测，所检测的矿石粉尘比石英危险性小、作用慢，但会导致进行性纤维化。体内生化实验无法提供肺组织可能受到的损伤类型的信息；矿石、膨润土和煤的数据彼此无差异。为了确定石英引起的典型生化变化，通过测定肺重量以及磷脂、磷脂酰胆碱和羟脯氨酸含量，我们获得了最有价值的信息。
