Schindhelm Roger K, Alssema Marjan, Scheffer Peter G, Diamant Michaela, Dekker Jacqueline M, Barto Rob, Nijpels Giel, Kostense Piet J, Heine Robert J, Schalkwijk Casper G, Teerlink Tom
Department of Endocrinology, Vrije University Medical Center, Amsterdam, The Netherlands.
Diabetes Care. 2007 Jul;30(7):1789-94. doi: 10.2337/dc06-2585. Epub 2007 Apr 27.
We studied acute changes in markers of glycoxidative and lipoxidative stress, including oxidized LDL, N(epsilon)-(carboxyethyl)-lysine (CEL), N(epsilon)-(carboxymethyl)-lysine (CML), and 3-deoxyglucosone (3DG), following two consecutive meals.
Postmenopausal women (27 with normal glucose metabolism [NGM], 26 with type 2 diabetes) received two consecutive fat-rich meals and two consecutive carbohydrate-rich meals on two occasions. Glucose and triglyceride concentrations were measured at baseline and 1, 2, 4, 6, and 8 h following breakfast; lunch was given at 4 h. Oxidized LDL-to-LDL cholesterol ratio, CEL, CML, and 3DG were measured at baseline and at 8 h.
Fasting oxidized LDL-to-LDL cholesterol ratio, 3DG, and CML were higher in women with type 2 diabetes compared with women with NGM and were comparable to the postprandial values at 8 h in NGM. Postprandial rises in the oxidized LDL-to-LDL cholesterol ratio and 3DG were similar in both groups. However, the oxidized LDL-to-LDL cholesterol ratio increased more after the fat-rich meals, whereas CML and 3DG increased more after the carbohydrate-rich meals. After the fat-rich meals, the increase in the oxidized LDL-to-LDL cholesterol ratio correlated with postprandial triglycerides, whereas the increase in 3DG was correlated with postprandial glucose.
The acute changes in markers of glycoxidative and lipoxidative stress in both type 2 diabetes and NGM suggest that postabsorptive oxidative stress may partly underlie the association of postprandial derangements and cardiovascular risk.
我们研究了连续两餐之后糖氧化应激和脂氧化应激标志物的急性变化，这些标志物包括氧化型低密度脂蛋白、N-ε-（羧乙基）-赖氨酸（CEL）、N-ε-（羧甲基）-赖氨酸（CML）和3-脱氧葡萄糖酮（3DG）。
绝经后女性（27例葡萄糖代谢正常[NGM]，26例2型糖尿病患者）分两次接受连续两顿富含脂肪的餐食和连续两顿富含碳水化合物的餐食。在早餐前及早餐后1、2、4、6和8小时测量血糖和甘油三酯浓度；4小时后给予午餐。在基线和8小时时测量氧化型低密度脂蛋白与低密度脂蛋白胆固醇比值、CEL、CML和3DG。
与葡萄糖代谢正常的女性相比，2型糖尿病女性的空腹氧化型低密度脂蛋白与低密度脂蛋白胆固醇比值、3DG和CML更高，且与葡萄糖代谢正常女性餐后8小时的值相当。两组餐后氧化型低密度脂蛋白与低密度脂蛋白胆固醇比值和3DG的升高相似。然而，富含脂肪的餐食后氧化型低密度脂蛋白与低密度脂蛋白胆固醇比值升高更多，而富含碳水化合物的餐食后CML和3DG升高更多。富含脂肪的餐食后，氧化型低密度脂蛋白与低密度脂蛋白胆固醇比值的升高与餐后甘油三酯相关，而3DG的升高与餐后血糖相关。
2型糖尿病和葡萄糖代谢正常者糖氧化应激和脂氧化应激标志物的急性变化表明，吸收后氧化应激可能部分是餐后代谢紊乱与心血管风险之间关联的基础。
