通过软光刻和两相流体流动形成阵列液滴及其在蛋白质结晶中的应用。
Formation of Arrayed Droplets by Soft Lithography and Two-Phase Fluid Flow, and Application in Protein Crystallization.
作者信息
Zheng Bo, Tice Joshua D, Ismagilov Rustem F
机构信息
Department of Chemistry, The University of Chicago, 5735 South Ellis Avenue, Chicago, IL 60637 (USA).
出版信息
Adv Mater. 2004 Aug 3;16(15):1365-1368. doi: 10.1002/adma.200400590.
Abstract
This paper presents an overview of our recent work on the use of soft lithography and two-phase fluid flow to form arrays of droplets. The crucial issues in the formation of stable arrays of droplets and alternating droplets of two sets of aqueous solutions include the geometry of the microchannels, the capillary number, and the water fraction of the system. Glass capillaries could be coupled to the PDMS microchannels and droplets could be transferred into glass capillaries for long-term storage. The arrays of droplets have been applied to screen the conditions for protein crystallization with microbatch and vapor diffusion techniques.
摘要
本文概述了我们最近关于使用软光刻和两相流体流动来形成液滴阵列的工作。形成两组水溶液的稳定液滴阵列和交替液滴的关键问题包括微通道的几何形状、毛细管数和系统的水分数。玻璃毛细管可以与聚二甲基硅氧烷（PDMS）微通道耦合，并且液滴可以转移到玻璃毛细管中进行长期储存。液滴阵列已应用于通过微量分批和气相扩散技术筛选蛋白质结晶的条件。
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