Liu Xiaozhen, Niu Hao, Li Qiang, Gu Pengfei
School of Biological Science and Technology, University of Jinan, Jinan, 250022 People's Republic of China.
3 Biotech. 2019 Mar;9(3):85. doi: 10.1007/s13205-019-1619-6. Epub 2019 Feb 15.
As one of the three proteinogenic aromatic amino acids, l-phenylalanine is widely applied in the food, chemical and pharmaceutical industries, especially in production of the low-calorie sweetener aspartame. Microbial production of l-phenylalanine has become attractive as it possesses the advantages of environmental friendliness, low cost, and feedstock renewability. With the progress of metabolic engineering, systems biology and synthetic biology, production of l-phenylalanine from glucose in with relatively high titer has been achieved by improving the intracellular levels of precursors, alleviating transcriptional repression and feedback inhibition of key enzymes, increasing the export of l-phenylalanine, engineering of global regulators, and overexpression of rate-limiting enzymes. In this review, successful metabolic engineering strategies for increasing l-phenylalanine accumulation from glucose in are described. In addition, perspectives for further improvement of production of l-phenylalanine are discussed.
作为三种蛋白质原性芳香族氨基酸之一，L-苯丙氨酸广泛应用于食品、化工和制药行业，尤其是在低热量甜味剂阿斯巴甜的生产中。L-苯丙氨酸的微生物生产因其具有环境友好、成本低和原料可再生等优点而备受关注。随着代谢工程、系统生物学和合成生物学的发展，通过提高前体物质的细胞内水平、减轻关键酶的转录抑制和反馈抑制、增加L-苯丙氨酸的输出、改造全局调控因子以及过表达限速酶，已经实现了从葡萄糖中以相对较高的滴度生产L-苯丙氨酸。在这篇综述中，描述了从葡萄糖中提高L-苯丙氨酸积累的成功代谢工程策略。此外，还讨论了进一步提高L-苯丙氨酸产量的前景。
