Henrissat B
Centre de Recherches sur les Macromolécules Végétales, C.N.R.S., Grenoble, France.
Biochem J. 1991 Dec 1;280 ( Pt 2)(Pt 2):309-16. doi: 10.1042/bj2800309.
The amino acid sequences of 301 glycosyl hydrolases and related enzymes have been compared. A total of 291 sequences corresponding to 39 EC entries could be classified into 35 families. Only ten sequences (less than 5% of the sample) could not be assigned to any family. With the sequences available for this analysis, 18 families were found to be monospecific (containing only one EC number) and 17 were found to be polyspecific (containing at least two EC numbers). Implications on the folding characteristics and mechanism of action of these enzymes and on the evolution of carbohydrate metabolism are discussed. With the steady increase in sequence and structural data, it is suggested that the enzyme classification system should perhaps be revised.
已对301种糖基水解酶及相关酶的氨基酸序列进行了比较。对应于39个酶委员会（EC）条目的总共291个序列可被分为35个家族。只有10个序列（占样本不到5%）无法归入任何家族。利用可用于此分析的序列，发现18个家族是单特异性的（仅包含一个EC编号），17个家族是多特异性的（包含至少两个EC编号）。讨论了这些酶的折叠特征、作用机制以及碳水化合物代谢进化方面的影响。随着序列和结构数据的不断增加，有人建议或许应该修订酶分类系统。
