Ma Ching-Man, Hon Chung-Chau, Lam Tsan-Yuk, Li Vince Yik-Yeung, Wong Creany Ka-Wai, de Oliveira Tulio, Leung Frederick Chi-Ching
Department of Zoology, The University of Hong Kong, Hong Kong SAR.
Department of Zoology, The University of Oxford, Oxford OX1 3PS, UK.
J Gen Virol. 2007 Jun;88(Pt 6):1733-1737. doi: 10.1099/vir.0.82629-0.
Porcine circovirus type 2 (PCV2) belongs to the family Circoviridae, and is the causative agent of post-weaning multisystemic wasting syndrome (PMWS) in pigs. In this study, phylogenetic analyses of three complete PCV2 genomic sequences from Hong Kong suggest that natural recombination happened among different lineages of PCV2. A preliminary investigation of the parental strains of these potential recombinants was carried out using bootscanning. Statistical significance of this recombination event was tested and positions of the potential recombination breakpoints were estimated in a maximum-likelihood framework. The recombinant breakpoints were estimated to be located within the origin of replication (ori) and replicase (rep) gene of PCV2. Interestingly, several GenBank sequences of PCV2 in mainland China were found to have a recombination pattern similar to that of the potential PCV2 recombinants from Hong Kong, implying that this recombinant genotype might already be widespread within mainland China.
猪圆环病毒2型（PCV2）属于圆环病毒科，是猪断奶后多系统消耗综合征（PMWS）的病原体。在本研究中，对来自香港的三个完整PCV2基因组序列进行系统发育分析，结果表明PCV2的不同谱系之间发生了自然重组。使用bootscanning对这些潜在重组体的亲本菌株进行了初步调查。在最大似然框架下测试了该重组事件的统计学显著性，并估计了潜在重组断点的位置。估计重组断点位于PCV2的复制起点（ori）和复制酶（rep）基因内。有趣的是，发现中国大陆的几个PCV2 GenBank序列具有与来自香港的潜在PCV2重组体相似的重组模式，这意味着这种重组基因型可能已经在中国大陆广泛传播。
