Wright Woodring E, Shay Jerry W
Department of Cell Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9039, USA.
Nat Protoc. 2006;1(4):2088-90. doi: 10.1038/nprot.2006.374.
Although the evidence is overwhelming that ambient oxygen is at least somewhat damaging to most normal cells in culture, the expense and effort involved has resulted in few laboratories growing their cells under physiological oxygen conditions. We here describe how to produce, from commercially available plastic wide-mouth jars, very simple gas-tight containers that can be flushed with prepared gas mixtures to produce low-oxygen environments for standard cell culture. This permits any laboratory to easily try the effects of physiological oxygen on their system without the need for dedicated incubators and substantial monetary investments.
尽管有压倒性的证据表明环境氧气对培养中的大多数正常细胞至少有一定程度的损害，但由于涉及的费用和精力，很少有实验室在生理氧气条件下培养细胞。我们在此描述如何用市售的塑料广口瓶制作非常简单的气密容器，这些容器可以用制备好的气体混合物冲洗，以产生用于标准细胞培养的低氧环境。这使得任何实验室都能轻松尝试生理氧气对其系统的影响，而无需专用培养箱和大量资金投入。
