Dei-Cas E, Dujardin L, Ribeiro Pinto M E, Ajana F, Fruit J, Poulain D, Camus D, Vernes A
Laboratoire de Parasitologie, Centre Hospitalier Universitaire, Lille, France.
Mycoses. 1991 Mar-Apr;34(3-4):167-72. doi: 10.1111/j.1439-0507.1991.tb00638.x.
The fungal growth of clinical yeast isolates and of VW32 clone of Candida albicans were measured in vitro using a liquid-phase turbidimetric system (Bioscreen from Labsystems, France) in defined conditions. Cultures were performed in Shadomy's liquid medium and the fungal growth automatically evaluated every 10 minutes for 24 hours using various concentrations of drugs. The system made it possible to test 200 culture samples in one experiment. Yeast sensitivity to drugs was also measured by using our routine semi-automatic turbidimetric system. We observed that kinetic patterns of activity of each antifungal agent were typical. The in vitro tests showed that of 927 clinical yeast isolates 99.2% were sensitive to amphotericin B, 94.4% to 5-fluorocytosine and 69.7% to ketoconazole.
在特定条件下，使用液相比浊系统（法国 Labsystems 公司的 Bioscreen）对临床酵母分离株和白色念珠菌 VW32 克隆的真菌生长进行体外测定。培养在沙多米液体培养基中进行，使用不同浓度的药物，每 10 分钟自动评估真菌生长情况，持续 24 小时。该系统在一次实验中能够检测 200 个培养样本。酵母对药物的敏感性也使用我们常规的半自动比浊系统进行测定。我们观察到每种抗真菌剂的活性动力学模式都是典型的。体外试验表明，在 927 株临床酵母分离株中，99.2% 对两性霉素 B 敏感，94.4% 对 5-氟胞嘧啶敏感，69.7% 对酮康唑敏感。
