Conrad Michael N, Lee Chih-Ying, Wilkerson Joseph L, Dresser Michael E
Program in Molecular, Cell and Developmental Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA.
Proc Natl Acad Sci U S A. 2007 May 22;104(21):8863-8. doi: 10.1073/pnas.0606165104. Epub 2007 May 10.
In meiotic prophase, telomeres associate with the nuclear envelope and accumulate adjacent to the centrosome/spindle pole to form the chromosome bouquet, a well conserved event that in Saccharomyces cerevisiae requires the meiotic telomere protein Ndj1p. Ndj1p interacts with Mps3p, a nuclear envelope SUN domain protein that is required for spindle pole body duplication and for sister chromatid cohesion. Removal of the Ndj1p-interaction domain from MPS3 creates an ndj1 Delta-like separation-of-function allele, and Ndj1p and Mps3p are codependent for stable association with the telomeres. SUN domain proteins are found in the nuclear envelope across phyla and are implicated in mediating interactions between the interior of the nucleus and the cytoskeleton. Our observations indicate a general mechanism for meiotic telomere movements.
在减数分裂前期，端粒与核膜结合，并在中心体/纺锤体极附近聚集形成染色体花束，这是一个高度保守的事件，在酿酒酵母中，该过程需要减数分裂端粒蛋白Ndj1p。Ndj1p与Mps3p相互作用，Mps3p是一种核膜SUN结构域蛋白，对于纺锤体极体复制和姐妹染色单体黏连是必需的。从MPS3中去除Ndj1p相互作用结构域会产生一个类似ndj1Δ的功能分离等位基因，并且Ndj1p和Mps3p对于与端粒的稳定结合是相互依赖的。SUN结构域蛋白存在于各门类生物的核膜中，并且参与介导细胞核内部与细胞骨架之间的相互作用。我们的观察结果表明了减数分裂端粒运动的一般机制。
