Madhyastha H K, Vatsala T M
Shri AMM Murugappa Chettiar Research Center, Photosynthesis and Energy Division, Taramani, Chennai, India.
Biomol Eng. 2007 Sep;24(3):301-5. doi: 10.1016/j.bioeng.2007.04.001. Epub 2007 Apr 19.
The present investigation makes a comparative investigation of individual light source on the different commercially important pigments in Spirulina fussiformis in photobioreactor culture condition. Continuous culture system was carried out throughout the experimental condition. Initially, seed culture, corresponding to 0.2 g/L on dry weight basis was cultivated in Zarrouks medium with different colored light source in reactor. Maximum daily biomass productivity, 0.8 g/L, 0.75 g/L and 0.69 g/L in white light (WL), blue light (BL) and green light (GL), respectively, conditions was noticed. Pigment content during WL treatment showed the highest accumulation (5.5 microg/mL) of chlorophyll whereas, other pigments roughly remained constant without much change, implying WL intensity is better for chlorophyll synthesis. On the other hand, chlorophyll and phycocyanin content gradually increased up to 7 microg/mL and 2 mg/mL, respectively, at BL intensity. The response to GL was negative to all pigments studied except for phycocyanin; in this case a highest production (2.5 mg/mL) was seen during 18 days experimental period. Additionally, when yellow light (YL) treatment experiments were conducted, the rate of production gradually decreased from 6th day onward in all pigments demonstrating the photobleaching effect of YL. The average rate of pigments production did not show significant accumulation in red light (RL) light treatment except phycoerythrin which showed an increasing trend of production. It is worth to mention here that higher light intensity is better for production of phycocyanin and phycoerythrin in Spirulina.
本研究对光生物反应器培养条件下，不同光源对钝顶螺旋藻中不同商业上重要色素的影响进行了比较研究。在整个实验过程中采用连续培养系统。最初，以干重计0.2 g/L的种子培养物在装有不同颜色光源的反应器中的扎鲁克培养基中培养。在白光（WL）、蓝光（BL）和绿光（GL）条件下，分别观察到最大日生物量生产率为0.8 g/L、0.75 g/L和0.69 g/L。在WL处理期间，色素含量显示叶绿素积累最高（5.5 μg/mL），而其他色素大致保持不变，变化不大，这意味着WL强度更有利于叶绿素合成。另一方面，在BL强度下，叶绿素和藻蓝蛋白含量分别逐渐增加至7 μg/mL和2 mg/mL。除藻蓝蛋白外，GL对所有研究的色素的反应均为负面；在这种情况下，在18天的实验期内观察到最高产量（2.5 mg/mL）。此外，当进行黄光（YL）处理实验时，从第6天起所有色素的产量逐渐下降，这表明YL的光漂白作用。除藻红蛋白产量呈上升趋势外，在红光（RL）处理中色素的平均产量没有显著积累。值得一提的是，较高的光照强度更有利于钝顶螺旋藻中藻蓝蛋白和藻红蛋白的生产。
