Terada T, Nakanuma Y, Ohta T, Nagakawa T, Motoo Y, Harada A, Hamato N, Inaba T
Second Department of Pathology, Kanazawa University School of Medicine, Japan.
Virchows Arch A Pathol Anat Histopathol. 1991;419(6):499-504. doi: 10.1007/BF01650679.
Epithelial cells of several types of hepatic cysts were examined by mucin histochemistry and immunohistochemically. There were some differences in mucus and antigenic expression among the hepatic cysts examined. Epithelial cells of non-parasitic simple cysts and adult-type polycystic liver showed similar mucin-histochemical and immunohistochemical features, and were characterized by little mucin and weak immunoreactivities to several antibodies examined. Epithelial cells of hepatic hilar cysts were characterized by much mucin and moderate immunoreactivities to carbohydrate antigen 19-9 (CA 19-9), carcinoembryonic antigen (CEA) and epithelial membrane antigen (EMA). Epithelial cells of ciliated hepatic foregut cysts were characterized by much mucin and immunoreactivities to actin and tubulin which were positive in cilia. Epithelial cells of biliary cystadenoma were characterized by much mucin and moderate to strong immunoreactivities to cytokeratins CAM5.2 and AE1 and 3 as well as to CA 19-9, CEA, EMA and DU-PAN-2. Epithelial cells of biliary cyst-adenocarcinoma were characterized by much mucin and moderate to strong immunoreactivities to cytokeratins CAM5.2 and AE1 and 3 as well as to CA 19-9, CEA, EMA and DU-PAN-2. These differences in epithelial mucus and antigenic expression among several types of hepatic cysts may reflect differences in their origin and biological characteristics. These differences may be helpful in the differential diagnosis of hepatic cysts in small biopsy specimens.
通过黏蛋白组织化学和免疫组织化学方法对几种肝囊肿的上皮细胞进行了检查。在所检查的肝囊肿中，黏液和抗原表达存在一些差异。非寄生虫性单纯囊肿和成人型多囊肝的上皮细胞表现出相似的黏蛋白组织化学和免疫组织化学特征，其特点是黏蛋白含量少，对几种检测抗体的免疫反应弱。肝门部囊肿的上皮细胞特点是黏蛋白含量多，对糖类抗原19-9（CA 19-9）、癌胚抗原（CEA）和上皮膜抗原（EMA）有中度免疫反应。纤毛肝前肠囊肿的上皮细胞特点是黏蛋白含量多，对肌动蛋白和微管蛋白有免疫反应，而这些蛋白在纤毛中呈阳性。胆管囊腺瘤的上皮细胞特点是黏蛋白含量多，对细胞角蛋白CAM5.2、AE1和3以及CA 19-9、CEA、EMA和DU-PAN-2有中度至强免疫反应。胆管囊腺癌的上皮细胞特点是黏蛋白含量多，对细胞角蛋白CAM5.2、AE1和3以及CA 19-9、CEA、EMA和DU-PAN-2有中度至强免疫反应。几种肝囊肿上皮黏液和抗原表达的这些差异可能反映了它们起源和生物学特性的差异。这些差异可能有助于小活检标本中肝囊肿的鉴别诊断。
