Steigert J, Grumann M, Brenner T, Riegger L, Harter J, Zengerle R, Ducrée J
University of Freiburg-IMTEK, Department of Microsystems Engineering, Laboratory for MEMS Applications, Georges-Koehler-Allee 106, D-79110 Freiburg, Germany.
Lab Chip. 2006 Aug;6(8):1040-4. doi: 10.1039/b607051p. Epub 2006 Jun 5.
We present a novel microfluidic concept to enable a fast colorimetric alcohol assay from a single droplet of whole blood. The reduced turn-around time of 150 seconds is, on the one hand, achieved by a full process integration including metering, mixing with reagents, and sedimentation of cellular constituents. On the other hand, our novel total internal reflection (TIR) scheme allows to monitor the increase of the absorbance values in real-time. Thus, the saturation values can be predicted accurately based on an extrapolation of real-time measurements acquired during a 100 second initial period of rotation. Additionally, we present a metering structure to define nanolitre sample volumes at a coefficient of variation (CV) below 5%.
我们提出了一种新颖的微流体概念，可实现从单滴全血中快速进行比色酒精检测。一方面，通过包括计量、与试剂混合以及细胞成分沉淀在内的全流程整合，实现了150秒的缩短周转时间。另一方面，我们新颖的全内反射（TIR）方案允许实时监测吸光度值的增加。因此，基于在100秒初始旋转期间获取的实时测量值的外推，可以准确预测饱和度值。此外，我们还提出了一种计量结构，以在变异系数（CV）低于5%的情况下定义纳升级的样品体积。
