Guan Bing, Zhu Dongdong, Dong Zhen, Yang Zhanquan
Department of Otorhinolaryngology-Head and Neck Surgery, Clinical Hospital, Yangzhou University, Yangzhou, 225001, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2007 Mar;21(6):269-72.
To detect expression level and distribution of aquaporin 1 (AQP1) in laryngeal carcinoma tissues and try to find out the significance about the mechanism of AQP1 in the invasion of laryngeal carcinoma.
Twenty cases of laryngeal carcinoma tissues and 15 cases of normal tissues beside tumors were acquired immediately after surgery. The expression and distribution of aquaporin 1 in laryngeal carcinoma were examined by RT-PCR, western blot analysis and immunohistochemical technique.
AQP1 expresses on the capillary endothelia of laryngeal mucous lamina propria and laryngeal mucous glands of normal tissues and mainly on the capillary endothelia cells, tumor cells and cancer nests of laryngeal carcinoma tissues. AQP1 mRNA and protein expression level in laryngeal tumor tissues is remarkably stronger than that in normal tissues beside tumors.
The expression of AQP1 was increased in laryngeal carcinoma tissues over normal laryngeal tissues. It suggests that the mechanism of AQP1 in laryngeal carcinoma etiopathogenesis needs to be studied.
检测水通道蛋白1（AQP1）在喉癌组织中的表达水平及分布情况，试图找出AQP1在喉癌侵袭机制中的意义。
手术切除后立即获取20例喉癌组织及15例肿瘤旁正常组织。采用逆转录-聚合酶链反应（RT-PCR）、蛋白质免疫印迹分析及免疫组织化学技术检测喉癌中AQP1的表达及分布。
AQP1在正常组织的喉黏膜固有层毛细血管内皮及喉黏液腺表达，在喉癌组织中主要表达于毛细血管内皮细胞、肿瘤细胞及癌巢。喉癌组织中AQP1 mRNA及蛋白表达水平显著高于肿瘤旁正常组织。
喉癌组织中AQP1的表达高于正常喉组织。提示需要研究AQP1在喉癌发病机制中的作用机制。
