Tu Z, Bozorgzadeh A, Crispe I N, Orloff M S
The Division of Solid Organ Transplantation and Hepatobiliary Surgery, Department of Surgery, University of Rochester Medical Center, Rochester, NY 14642, USA.
Clin Exp Immunol. 2007 Jul;149(1):186-93. doi: 10.1111/j.1365-2249.2007.03415.x. Epub 2007 May 18.
The immune tolerance induced by the liver as an allograft is difficult to reconcile with the evidence that the liver selectively accumulates activated T cells from the circulation. However, much of this information is based on murine liver lymphocytes that were isolated using enzymatic digestion. In the present study we made use of a novel resource, the lymphocytes isolated during the perfusion of living donor liver lobe prior to transplantation. These healthy human liver lymphocytes displayed surface markers indicating a high degree of activation of natural killer cells, CD56(+) T cells, CD4(+) T cells and CD8(+) T cells. These properties were independent of enzymatic treatment or the details of cell isolation. We conclude that the healthy human liver is a site of intense immunological activity.
肝脏作为同种异体移植器官所诱导的免疫耐受，很难与肝脏能从循环中选择性聚集活化T细胞这一证据相协调。然而，这些信息大多基于通过酶消化分离得到的小鼠肝脏淋巴细胞。在本研究中，我们利用了一种新资源，即移植前在活体供肝叶灌注过程中分离得到的淋巴细胞。这些健康的人肝脏淋巴细胞显示出表面标志物，表明自然杀伤细胞、CD56(+) T细胞、CD4(+) T细胞和CD8(+) T细胞高度活化。这些特性与酶处理或细胞分离细节无关。我们得出结论，健康的人肝脏是一个免疫活动强烈的场所。
