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HBV 感染肝脏的高多重化二维成像质谱细胞分析法。

Highly multiplexed 2-dimensional imaging mass cytometry analysis of HBV-infected liver.

机构信息

Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA.

Medical Research, The Corporal Michael J. Crescenz VA Medical Center, Philadelphia, Pennsylvania, USA.

出版信息

JCI Insight. 2021 Apr 8;6(7):146883. doi: 10.1172/jci.insight.146883.

DOI:10.1172/jci.insight.146883
PMID:33621209
原文链接:
https://pmc.ncbi.nlm.nih.gov/articles/PMC8119221/
Abstract

Studies of human hepatitis B virus (HBV) immune pathogenesis are hampered by limited access to liver tissues and technologies for detailed analyses. Here, utilizing imaging mass cytometry (IMC) to simultaneously detect 30 immune, viral, and structural markers in liver biopsies from patients with hepatitis B e antigen+ (HBeAg+) chronic hepatitis B, we provide potentially novel comprehensive visualization, quantitation, and phenotypic characterizations of hepatic adaptive and innate immune subsets that correlated with hepatocellular injury, histological fibrosis, and age. We further show marked correlations between adaptive and innate immune cell frequencies and phenotype, highlighting complex immune interactions within the hepatic microenvironment with relevance to HBV pathogenesis.

摘要

利用成像质谱细胞术（IMC）同时检测 30 种免疫、病毒和结构标志物，我们对 HBeAg+慢性乙型肝炎患者的肝活检进行了分析，为肝内适应性和固有免疫亚群提供了潜在的新的全面可视化、定量和表型特征，这些特征与肝细胞损伤、组织纤维化和年龄相关。我们还进一步显示了适应性和固有免疫细胞频率和表型之间的显著相关性，突出了肝微环境中与乙型肝炎病毒发病机制相关的复杂免疫相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1665/8119221/60997be067f3/jciinsight-6-146883-g224.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1665/8119221/a37583036713/jciinsight-6-146883-g220.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1665/8119221/73ff1c64f02d/jciinsight-6-146883-g221.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1665/8119221/b8a6eb38d5a4/jciinsight-6-146883-g222.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1665/8119221/9337facfb178/jciinsight-6-146883-g223.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1665/8119221/60997be067f3/jciinsight-6-146883-g224.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1665/8119221/a37583036713/jciinsight-6-146883-g220.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1665/8119221/73ff1c64f02d/jciinsight-6-146883-g221.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1665/8119221/b8a6eb38d5a4/jciinsight-6-146883-g222.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1665/8119221/9337facfb178/jciinsight-6-146883-g223.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1665/8119221/60997be067f3/jciinsight-6-146883-g224.jpg

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2
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4
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5
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6
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