Russell A S, Kaiser J, Lao V S
J Immunol Methods. 1976;9(3-4):273-9. doi: 10.1016/0022-1759(76)90202-7.
The cell mediated immune response to herpes simplex virus was assessed by a modification of the leukocyte migration inhibition test, developed to increase its sensitivity and correlated with the in vitro stimulation of lymphocytes. The introduction of a preincubation step allowed us to demonstrate significant inhibition of leukocyte migration in 15 out of 24 subjects susceptible to recurrent herpes labialis. This was not present in any of the 10 subjects without such infections. However lymphocytes from all 24 subjects demonstrated stimulation in response to herpes virus antigen. The degrees of lymphocyte stimulation and migration inhibition in the susceptible subjects were well correlated and do not support the suggestion that there is a focal defect in this aspect of the cell mediated immune response to herpes simplex virus in those patients.
通过对白细胞迁移抑制试验进行改良来评估针对单纯疱疹病毒的细胞介导免疫反应，该改良旨在提高其敏感性并与淋巴细胞的体外刺激相关联。预孵育步骤的引入使我们能够在24名易患复发性唇疱疹的受试者中的15名中证明白细胞迁移受到显著抑制。在10名无此类感染的受试者中，均未出现这种情况。然而，所有24名受试者的淋巴细胞在接触疱疹病毒抗原后均表现出刺激反应。易感受试者中淋巴细胞刺激程度和迁移抑制程度具有良好的相关性，并不支持以下观点：在这些患者中，针对单纯疱疹病毒的细胞介导免疫反应的这一方面存在局灶性缺陷。
