Maity Sulata, Roy Sonali, Chaudhury Shibani, Bhattacharya Shelley
Centre for Environmental Studies, Department of Zoology, Siksha-Bhavana, Visva-Bharati, Santiniketan 731 235, India.
Environ Pollut. 2008 Jan;151(1):1-7. doi: 10.1016/j.envpol.2007.03.005. Epub 2007 May 23.
The aim of this study was to provide fundamental data on the biochemical analysis of antioxidant defences in the earthworm exposed to low levels (75, 150, 300 mg kg(-1) soil) of Pb and Zn. In order to attain this objective, adult Lampito mauritii were exposed to different doses of Pb and Zn separately for 28 days and the concentrations of oxidized and reduced glutathione, activities of glutathione-S-transferase, glutathione peroxidase and glutathione reductase were assessed. Dose-dependent perturbations were observed in the glutathione-glutathione-S-transferase system and other antioxidant enzymes during the early phase of the exposure to Pb. In the Zn exposed earthworm, the glutathione-glutathione-S-transferase system remained stable and the stimulation of glutathione peroxidase and glutathione reductase activities occurred significantly only on day 14 at 300 mg Zn kg(-1). It is concluded that the antioxidants are directly involved in the adaptive response of Lampito mauritii for survival in metal contaminated soil.
本研究的目的是提供蚯蚓暴露于低水平（75、150、300 mg kg⁻¹土壤）铅和锌时抗氧化防御生化分析的基础数据。为实现这一目标，将成年毛里求斯腔蚓分别暴露于不同剂量的铅和锌中28天，并评估氧化型和还原型谷胱甘肽的浓度、谷胱甘肽-S-转移酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性。在暴露于铅的早期阶段，谷胱甘肽-谷胱甘肽-S-转移酶系统和其他抗氧化酶中观察到剂量依赖性扰动。在暴露于锌的蚯蚓中，谷胱甘肽-谷胱甘肽-S-转移酶系统保持稳定，仅在第14天，当锌含量为300 mg kg⁻¹时，谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性才显著增强。得出的结论是，抗氧化剂直接参与毛里求斯腔蚓在金属污染土壤中生存的适应性反应。
