• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

α-琼胶酶定义了一个新的糖苷水解酶家族,与β-琼胶酶家族不同。

Alpha-agarases define a new family of glycoside hydrolases, distinct from beta-agarase families.

作者信息

Flament Didier, Barbeyron Tristan, Jam Murielle, Potin Philippe, Czjzek Mirjam, Kloareg Bernard, Michel Gurvan

机构信息

Centre National de la Recherche Scientifique, Université Pierre et Marie Curie-Paris6, Unité Mixte de Recherche 7139, Station Biologique, F-29682 Roscoff Cedex, Bretagne, France.

出版信息

Appl Environ Microbiol. 2007 Jul;73(14):4691-4. doi: 10.1128/AEM.00496-07. Epub 2007 May 18.

DOI:10.1128/AEM.00496-07
PMID:17513582
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1932826/
Abstract

The gene encoding the alpha-agarase from "Alteromonas agarilytica" (proposed name) has been cloned and sequenced. The gene product (154 kDa) is unrelated to beta-agarases and instead belongs to a new family of glycoside hydrolases (GH96). The alpha-agarase also displays a complex modularity, with the presence of five thrombospondin type 3 repeats and three carbohydrate-binding modules.

摘要

编码来自“嗜琼脂交替单胞菌”(暂定名)的α-琼脂酶的基因已被克隆和测序。该基因产物(154 kDa)与β-琼脂酶无关,而是属于糖苷水解酶的一个新家族(GH96)。α-琼脂酶还表现出复杂的模块性,存在五个血小板反应蛋白3型重复序列和三个碳水化合物结合模块。

相似文献

1
Alpha-agarases define a new family of glycoside hydrolases, distinct from beta-agarase families.α-琼胶酶定义了一个新的糖苷水解酶家族,与β-琼胶酶家族不同。
Appl Environ Microbiol. 2007 Jul;73(14):4691-4. doi: 10.1128/AEM.00496-07. Epub 2007 May 18.
2
Cloning, expression, and biochemical characterization of a novel GH16 β-agarase AgaG1 from Alteromonas sp. GNUM-1.从海洋细菌 Alteromonas sp. GNUM-1 中克隆、表达和生化表征一种新型 GH16β-琼脂酶 AgaG1。
Appl Microbiol Biotechnol. 2014 May;98(10):4545-55. doi: 10.1007/s00253-014-5510-4. Epub 2014 Jan 26.
3
Enzymatic properties and nucleotide and amino acid sequences of a thermostable beta-agarase from a novel species of deep-sea Microbulbifer.来自深海微球藻新物种的一种耐热β-琼脂酶的酶学性质、核苷酸和氨基酸序列
Appl Microbiol Biotechnol. 2004 May;64(4):505-14. doi: 10.1007/s00253-004-1573-y. Epub 2004 Feb 20.
4
Cloning, expression, and characterization of a glycoside hydrolase family 86 beta-agarase from a deep-sea Microbulbifer-like isolate.来自深海类微小杆菌分离株的糖苷水解酶家族86 β-琼胶酶的克隆、表达及特性分析
Appl Microbiol Biotechnol. 2004 Dec;66(3):266-75. doi: 10.1007/s00253-004-1757-5.
5
Cloning, expression and characterization of a new agarase-encoding gene from marine Pseudoalteromonas sp.来自海洋假交替单胞菌属的一个新的琼脂酶编码基因的克隆、表达及特性分析
Biotechnol Lett. 2009 Oct;31(10):1565-70. doi: 10.1007/s10529-009-0042-1. Epub 2009 Jun 6.
6
Characterization of a novel β-agarase from an agar-degrading bacterium Catenovulum sp. X3.从琼脂降解菌 Catenovulum sp. X3 中鉴定一种新型 β-琼脂酶。
Appl Microbiol Biotechnol. 2013 Jun;97(11):4907-15. doi: 10.1007/s00253-012-4385-5. Epub 2012 Sep 20.
7
Cloning, expression, and characterization of a glycoside hydrolase family 50 beta-agarase from a marine Agarivorans isolate.从海洋琼胶降解菌分离物中克隆、表达及鉴定糖苷水解酶家族50的β-琼脂酶
Biotechnol Lett. 2006 Dec;28(23):1925-32. doi: 10.1007/s10529-006-9171-y. Epub 2006 Sep 23.
8
Cloning, expression, and characterization of a new beta-agarase from Vibrio sp. strain CN41.从弧菌 CN41 中克隆、表达和表征一种新型β-琼脂酶。
Appl Environ Microbiol. 2011 Oct;77(19):7077-9. doi: 10.1128/AEM.05364-11. Epub 2011 Aug 5.
9
Characterization of an α-agarase from Thalassomonas sp. LD5 and its hydrolysate.海洋硫杆菌 LD5 中 α-琼胶酶的特性及其水解产物分析。
Appl Microbiol Biotechnol. 2018 Mar;102(5):2203-2212. doi: 10.1007/s00253-018-8762-6. Epub 2018 Jan 20.
10
Gene cloning, expression, and characterization of a beta-agarase, agaB34,from Agarivorans albus YKW-34.来自白色食琼脂菌YKW-34的β-琼脂酶agaB34的基因克隆、表达及特性分析
J Microbiol Biotechnol. 2009 Mar;19(3):257-64.

引用本文的文献

1
Mining Arylsulfatase from Genome-Scale Metabolic Pathways of Pseudoalteromonas sp. SR43-6 and Its Agar-Based Desulfurization Applications.从假交替单胞菌属SR43-6的基因组规模代谢途径中挖掘芳基硫酸酯酶及其基于琼脂的脱硫应用
ACS Omega. 2025 Apr 22;10(17):18005-18016. doi: 10.1021/acsomega.5c01356. eCollection 2025 May 6.
2
Catalytic Mode and Product Specificity of an α-Agarase Reveal Its Direct Catalysis for the Production of Agarooligosaccharides.一种α-琼脂酶的催化模式和产物特异性揭示了其对琼脂寡糖生产的直接催化作用。
Foods. 2024 Jul 26;13(15):2351. doi: 10.3390/foods13152351.
3
Genome sequences of four agarolytic bacteria from the Bacteroidia and Gammaproteobacteria.来自拟杆菌纲和γ-变形菌纲的四种琼脂分解菌的基因组序列。
Microbiol Resour Announc. 2023 Nov 16;12(11):e0066723. doi: 10.1128/MRA.00667-23. Epub 2023 Oct 9.
4
Extracellular production of a thermostable Cellvibrio endolytic β-agarase in Escherichia coli for agarose liquefaction.在大肠杆菌中胞外生产用于琼脂糖液化的耐热性溶琼脂纤维杆菌内切β-琼脂酶。
AMB Express. 2023 May 5;13(1):42. doi: 10.1186/s13568-023-01551-w.
5
Enzymatic characterization of a novel recombinant 1,3-α-3,6-anhydro-L-galactosidase specific for neoagarobiose hydrolysis into monosaccharides.新型重组 1,3-α-3,6-脱水-L-半乳糖基酶对 neoagarobiose 水解成单糖的酶学特性分析。
Appl Microbiol Biotechnol. 2021 Jun;105(11):4621-4634. doi: 10.1007/s00253-021-11341-8. Epub 2021 May 31.
6
A Novel Auxiliary Agarolytic Pathway Expands Metabolic Versatility in the Agar-Degrading Marine Bacterium Colwellia echini A3.一种新型辅助琼脂分解途径扩展了琼脂降解海洋细菌海胆科氏菌A3的代谢多样性。
Appl Environ Microbiol. 2021 May 26;87(12):e0023021. doi: 10.1128/AEM.00230-21.
7
Characterization and Application of a Recombinant Exolytic GH50A β-Agarase from sp. KY-GH-1 for Enzymatic Production of Neoagarobiose from Agarose.来自sp. KY-GH-1的重组外切型GH50Aβ-琼脂酶的表征及其在从琼脂糖酶法生产新琼脂二糖中的应用
ACS Omega. 2020 Nov 5;5(45):29453-29464. doi: 10.1021/acsomega.0c04390. eCollection 2020 Nov 17.
8
A Multifunctional Polysaccharide Utilization Gene Cluster in Encodes Enzymes for the Complete Degradation of κ-Carrageenan, ι-Carrageenan, and Hybrid β/κ-Carrageenan.一个多功能多糖利用基因簇在 中编码了用于完全降解 κ-卡拉胶、ι-卡拉胶和混合 β/κ-卡拉胶的酶。
mSphere. 2020 Jan 8;5(1):e00792-19. doi: 10.1128/mSphere.00792-19.
9
Whole genome sequence of a freshwater agar-degrading bacterium sp. KY-GH-1.一株淡水琼脂降解菌KY-GH-1的全基因组序列
Biotechnol Rep (Amst). 2019 May 18;23:e00346. doi: 10.1016/j.btre.2019.e00346. eCollection 2019 Sep.
10
Biochemical Characteristics and Substrate Degradation Pattern of a Novel Exo-Type β-Agarase from the Polysaccharide-Degrading Marine Bacterium Flammeovirga sp. Strain MY04.来自多糖降解海洋细菌火焰弧菌属菌株MY04的一种新型外切型β-琼脂酶的生化特性及底物降解模式
Appl Environ Microbiol. 2016 Jul 29;82(16):4944-54. doi: 10.1128/AEM.00393-16. Print 2016 Aug 15.

本文引用的文献

1
Cloning and biochemical characterization of the fucanase FcnA: definition of a novel glycoside hydrolase family specific for sulfated fucans.岩藻聚糖酶FcnA的克隆与生化特性:对一种特异于硫酸化岩藻聚糖的新型糖苷水解酶家族的定义
Glycobiology. 2006 Nov;16(11):1021-32. doi: 10.1093/glycob/cwl029. Epub 2006 Jul 31.
2
Family 6 carbohydrate binding modules in beta-agarases display exquisite selectivity for the non-reducing termini of agarose chains.β-琼胶酶中的第6家族碳水化合物结合模块对琼脂糖链的非还原末端表现出极高的选择性。
J Biol Chem. 2006 Jun 23;281(25):17099-17107. doi: 10.1074/jbc.M600702200. Epub 2006 Apr 6.
3
Bioconversion of red seaweed galactans: a focus on bacterial agarases and carrageenases.红海藻半乳聚糖的生物转化:聚焦于细菌琼脂酶和卡拉胶酶。
Appl Microbiol Biotechnol. 2006 Jun;71(1):23-33. doi: 10.1007/s00253-006-0377-7. Epub 2006 Mar 21.
4
InterProScan: protein domains identifier.InterProScan:蛋白质结构域识别工具。
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W116-20. doi: 10.1093/nar/gki442.
5
Structure of a full length psychrophilic cellulase from Pseudoalteromonas haloplanktis revealed by X-ray diffraction and small angle X-ray scattering.通过X射线衍射和小角X射线散射揭示的嗜冷性假交替单胞菌全长纤维素酶的结构
J Mol Biol. 2005 May 20;348(5):1211-24. doi: 10.1016/j.jmb.2005.03.026. Epub 2005 Mar 25.
6
The endo-beta-agarases AgaA and AgaB from the marine bacterium Zobellia galactanivorans: two paralogue enzymes with different molecular organizations and catalytic behaviours.来自海洋细菌食半乳聚糖佐贝尔氏菌的内切β-琼脂糖酶AgaA和AgaB:两种具有不同分子结构和催化行为的旁系同源酶。
Biochem J. 2005 Feb 1;385(Pt 3):703-13. doi: 10.1042/BJ20041044.
7
Improved prediction of signal peptides: SignalP 3.0.信号肽预测的改进:SignalP 3.0
J Mol Biol. 2004 Jul 16;340(4):783-95. doi: 10.1016/j.jmb.2004.05.028.
8
Parallel substrate binding sites in a beta-agarase suggest a novel mode of action on double-helical agarose.β-琼脂酶中的平行底物结合位点表明其对双螺旋琼脂糖具有一种新的作用模式。
Structure. 2004 Apr;12(4):623-32. doi: 10.1016/j.str.2004.02.020.
9
Structure of a thrombospondin C-terminal fragment reveals a novel calcium core in the type 3 repeats.血小板反应蛋白C端片段的结构揭示了3型重复序列中的一种新型钙核心。
EMBO J. 2004 Mar 24;23(6):1223-33. doi: 10.1038/sj.emboj.7600166. Epub 2004 Mar 11.
10
The three-dimensional structures of two beta-agarases.两种β-琼脂糖酶的三维结构。
J Biol Chem. 2003 Nov 21;278(47):47171-80. doi: 10.1074/jbc.M308313200. Epub 2003 Sep 11.