Yang Jian, Tian Feng, Wang Zheng, Wang Qing, Zeng Yan-Jun, Chen Shi-Qian
Tianjin Institute of Medical Equipment, Tianjin 300161, People's Republic of China.
J Biomed Mater Res B Appl Biomater. 2008 Jan;84(1):131-7. doi: 10.1002/jbm.b.30853.
Comparative studies have been carried out among solid-state chitosan soliquoid, chitosan acetic acid physiological saline solution, and carboxymethyl chitosan physiological saline solution to discover the hemostatic effect of molecular weight (M(w)) and deacetylation degree (DA) of chitosan. It was found that solid-state chitosan and chitosan acetic acid physiological saline solution performed different hemostatic mechanisms. When blood mixed with chitosan acetic acid physiological saline solution, the erythrocytes aggregated and were deformed. The DA, especially a low DA, in the chitosan acetic acid physiological saline solution, had a significant effect on the unusual aggregation and deformation of erythrocytes, compared with the effect of M(w) within a range between 10(5) and 10(6). However, this phenomenon could not be observed in solid-state chitosan soliquoid. Solid-state chitosan with a low DA absorbed more platelets and was more hemostatic. Carboxymethyl chitosan physiological saline solution had nothing to do with the aggregation and deformation of erythrocytes but caused local rouleau. The values of thrombin time (TT), prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen concentration (FIB) were measured after the blood was mixed with solid-state chitosan soliquoid, chitosan acetic acid physiological saline solution, and carboxymethyl chitosan physiological saline solution, separately. The results demonstrated that coagulation factors might not be activated by them.
已对固态壳聚糖溶胶、壳聚糖乙酸生理盐水溶液和羧甲基壳聚糖生理盐水溶液进行了比较研究，以发现壳聚糖的分子量（M(w)）和脱乙酰度（DA）的止血效果。结果发现，固态壳聚糖和壳聚糖乙酸生理盐水溶液具有不同的止血机制。当血液与壳聚糖乙酸生理盐水溶液混合时，红细胞聚集并变形。壳聚糖乙酸生理盐水溶液中的DA，尤其是低DA，与红细胞的异常聚集和变形有显著关系，相比之下，在10(5)至10(6)范围内的M(w)的影响则较小。然而，在固态壳聚糖溶胶中未观察到这种现象。低DA的固态壳聚糖吸收更多血小板且止血效果更好。羧甲基壳聚糖生理盐水溶液与红细胞的聚集和变形无关，但会导致局部红细胞缗钱状形成。分别将血液与固态壳聚糖溶胶、壳聚糖乙酸生理盐水溶液和羧甲基壳聚糖生理盐水溶液混合后，测量凝血酶时间（TT）、凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）和纤维蛋白原浓度（FIB）的值。结果表明，它们可能不会激活凝血因子。
