Faculty of Chemistry and Environmental Science, Guangdong Ocean University, Zhanjiang 524088, China.
Molecules. 2018 Nov 30;23(12):3147. doi: 10.3390/molecules23123147.
Hemorrhea is one of the major problems in war, trauma care, and surgical operation that threaten the life of the injured and patients. As a novel polymeric hemostatic agent, biodegradable chitosan can stop bleeding through a variety of approaches. In this paper, chitosan with various molecular parameters was prepared from chitin as raw material through deacetylation, oxidative degradation, hydrophilic modification, and salt formation reactions. The influence of different polymer parameters on the hemostatic effects of chitosan was investigated by in vitro coagulation time and dynamic coagulation assay. The results showed that when the molecular weights were high (10⁵⁻10⁶) and approximate, the coagulation effect of chitosan improved with a decrease of the deacetylation degree and achieved a prominent level in a moderate degree of deacetylation (68.36%). With the same degree of deacetylation, the higher the molecular weight of chitosan, the better the procoagulant effect. The substituent derivatives and acid salts of chitosan showed significant procoagulant effects, especially the acid salts of chitosan. In addition, the hemostasis mechanism of chitosan with various parameters was preliminarily explored by analyzing the plasma recalcification time (PRT). The efforts in this paper laid a basis for further study of the structure⁻activity relationship and the mechanism of chitosan hemostasis.
出血是战争、创伤护理和外科手术中的主要问题之一，威胁着伤员和患者的生命。作为一种新型的聚合止血剂，可生物降解的壳聚糖可以通过多种途径止血。本文以甲壳素为原料，通过脱乙酰化、氧化降解、亲水性改性和盐形成反应，制备了具有不同分子参数的壳聚糖。通过体外凝血时间和动态凝血试验研究了不同聚合物参数对壳聚糖止血效果的影响。结果表明，当分子量较高（10⁵⁻10⁶）且相近时，壳聚糖的凝血效果随脱乙酰度的降低而提高，并在中度脱乙酰度（68.36%）时达到显著水平。在相同脱乙酰度下，壳聚糖的分子量越高，促凝效果越好。壳聚糖的取代衍生物和酸式盐表现出显著的促凝作用，尤其是壳聚糖的酸式盐。此外，通过分析血浆再钙化时间（PRT），初步探讨了具有不同参数的壳聚糖的止血机制。本文的研究工作为进一步研究壳聚糖止血的结构-活性关系和机制奠定了基础。
