Trinh Quang Duy, Nguyen Tuan Anh, Phan Tung Gia, Khamrin Pattara, Yan Hainian, Hoang Phuc Le, Maneekarn Niwat, Li Yan, Yagyu Fumihiro, Okitsu Shoko, Ushijima Hiroshi
Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, University of Tokyo, Japan.
J Med Virol. 2007 Jul;79(7):1009-16. doi: 10.1002/jmv.20920.
Over the last decade, rotavirus G1 has represented the most common genotype worldwide. Since 2000, the prevalence of rotavirus G1 has decreased in some countries such as Japan and China. To monitor the trend of the VP7 encoding gene of rotavirus G1, we performed a sequence analysis of 74 G1 rotavirus strains isolated in Japan, China, Thailand, and Vietnam during the period from 2002 to 2005. The phylogenetic tree showed that all of the studied G1 strains from the four countries clustered into lineage III, the same as the majority of the G1 strains isolated in China and Japan in 1990 and 1991. Examination of the deduced amino acid sequences of the G1 strains from China and Japan revealed an amino acid substitution at position 91 (Asn instead of Thr) in antigenic region A when compared to the G1 strains isolated in China and Japan in 1990, 1991, and global reference strains. For the G1 strains from Thailand and Vietnam, there were three amino acid substitutions, not belonging to any antigenic regions. The study showed that there have been no considerable changes of human rotavirus G1 isolated in Japan, China, Thailand, and Vietnam. Further studies need to be carried out for a better understanding of why such changes in the prevalence of rotavirus G1 occur in these countries.
在过去十年中，轮状病毒G1一直是全球最常见的基因型。自2000年以来，轮状病毒G1在一些国家（如日本和中国）的流行率有所下降。为了监测轮状病毒G1的VP7编码基因的变化趋势，我们对2002年至2005年期间在日本、中国、泰国和越南分离出的74株G1轮状病毒进行了序列分析。系统发育树显示，来自这四个国家的所有研究G1菌株都聚集在谱系III中，与1990年和1991年在中国和日本分离出的大多数G1菌株相同。对来自中国和日本的G1菌株推导的氨基酸序列进行检查发现，与1990年、1991年在中国和日本分离出的G1菌株以及全球参考菌株相比，抗原区域A的第91位氨基酸发生了取代（天冬酰胺取代苏氨酸）。对于来自泰国和越南的G1菌株，有三个氨基酸取代，不属于任何抗原区域。该研究表明，在日本、中国、泰国和越南分离出的人类轮状病毒G1没有发生显著变化。需要进一步开展研究，以更好地理解为什么轮状病毒G1在这些国家的流行率会出现这样的变化。
